Proteomics

Dataset Information

0

The VIR-associated proteins were identified by LC-MS/MS


ABSTRACT: 7-day-old Col-0 seedlings were collected, and 2 g of tissue was ground to a fine powder in liquid nitrogen and homogenized in 2 ml of lysis buffer (50 mM Tris-HCl, pH 7.4, 2.5 mM MgCl2, 100 mM KCl, 0.1% [v/v] Nonidet P-40, 1 μg/ml leupeptin, 1 μg/ml aprotonin, 0.5 mM phenylmethylsulfonyl fluoride, one tablet of Complete proteinase inhibitor [Roche], and 50 units/ml RNase OUT [Invitrogen]). The lysate was incubated on ice for 5 min and centrifuged at 13,000 g for 10 min at 4°C to pellet cell debris. Clarified lysates were subjected to immunoprecipitation with anti-VIR antibody or normal rabbit IgG (#2729, Cell Signaling Technology, USA) bound to protein A agarose (Sigma-Aldrich)

ORGANISM(S): Arabidopsis Thaliana

SUBMITTER: Honglei Jin  

PROVIDER: PXD038080 | iProX | Mon Nov 14 00:00:00 GMT 2022

REPOSITORIES: iProX

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Publications

N<sup>6</sup>-methyladenosine RNA modification regulates photosynthesis during photodamage in plants.

Zhang Man M   Zeng Yunping Y   Peng Rong R   Dong Jie J   Lan Yelin Y   Duan Sujuan S   Chang Zhenyi Z   Ren Jian J   Luo Guanzheng G   Liu Bing B   Růžička Kamil K   Zhao Kewei K   Wang Hong-Bin HB   Jin Hong-Lei HL  

Nature communications 20221202 1


N<sup>6</sup>-methyladenosine (m<sup>6</sup>A) modification of mRNAs affects many biological processes. However, the function of m<sup>6</sup>A in plant photosynthesis remains unknown. Here, we demonstrate that m<sup>6</sup>A modification is crucial for photosynthesis during photodamage caused by high light stress in plants. The m<sup>6</sup>A modification levels of numerous photosynthesis-related transcripts are changed after high light stress. We determine that the Arabidopsis m<sup>6</sup>A w  ...[more]

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