Dataset Information

Extracellular vesicles play a central role in the cerebral deep medullary vein reduction-associated brain atrophy

ABSTRACT: EVs were extracted from the serum of normal and DMV-reduction participants and compared their proteome profiles through a combination of liquid chromatography-tandem mass spectroscopy analysis and Tandem Mass Tag label quantitation analysis. In vitro assays were also conducted to access the impact of EVs on the reduction of vein number and brain atrophy, using serum-derived EVs to treat neurons, astrocytes, endothelial cells, and smooth muscle cells.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wei Ge

PROVIDER: PXD039873 | iProX | Fri Feb 03 00:00:00 GMT 2023

REPOSITORIES: iProX

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Publications

Extracellular Vesicles Play a Central Role in Cerebral Venous Disease-Associated Brain Atrophy.

Wang Jia-Yu JY Li Jing-Ying JY Luo Dan D Huang Mei-Ying MY Ao Dong-Hui DH Liu Xin-Nan XN Wang Xia X Ge Wei W Zhu Yi-Cheng YC

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 20230712 27

Cerebral venous abnormalities, distinct from traditional arterial diseases, have been linked to brain atrophy in a previous community-based cohort study, specifically in relation to the reduction of deep medullary veins (r-DMVs). To better understand the properties and biological functions of serum extracellular vesicles (EVs) in cerebral venous disease-associated brain atrophy, EVs are extracted from the serum of both participants with r-DMV and normal controls and analyzed their proteomic prof ...[more]

PMID: 37438638

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Project description:Circulating extracellular vesicles (EVs) contain molecular footprints - lipids, proteins, metabolites, RNA, and DNA - from their cell of origin and may provide non-invasive access for detection, characterization, and monitoring of diseases. Consequently, EV-associated RNA and proteins have gained widespread interest in developing liquid-biopsy assays for cancer. Yet, an integrative proteo-transcriptomic landscape of EVs and a direct comparison with their cell of origin remains relatively unexplored. Here, we report that EVs enrich for distinct molecular cargo and their proteo-transcriptome does not linearly correlate with their cell of origin. Our sub-cellular compartment analyses revealed that EV enrich 2-6 times more cytoskeletal and extracellular proteins, while their donor cells cargo 3-10 times more nuclear and mitochondrial proteins. EVs predominantly enrich (40-60%) for small RNA between ~15-200 nucleotides, which include miRNAs, siRNAs, tRNA, 5S rRNAs, and snRNAs, associated with cell differentiation, development, and Wnt signaling signatures. Finally, our integrated proteo-transcriptomic analyses reveal that EVs are enriched with specific small RNAs (RNY3, vtRNA, and MIRLET-7) and their complementary proteins (YBX1, IGF2BP2, SRSF1/2). These analyses suggest that RNA-protein complexes may constitute a functional interaction network inside EVs to protect and regulate access to EV-RNA. To ensure that our analyses are unbiased and independent of the cell of origin, EV isolation methods, site of experimentation, site of data generation, and methodologies applied for RNAseq and mass spectrometry, we have generated and curated comprehensive datasets of proteomics and transcriptomics from a total of 12 cancer cell lines, their EVs, and prostate cancer patientsÕ serum EVs (A detailed description is provided in Fig. 1). These combined analyses demonstrated that irrespective of the cell of origin and other aforementioned conditions, EVs enrich for distinct RNA and protein signatures that do not linearly correlate with their cell of origin.

Dataset Information

Extracellular vesicles play a central role in the cerebral deep medullary vein reduction-associated brain atrophy

Publications

Extracellular Vesicles Play a Central Role in Cerebral Venous Disease-Associated Brain Atrophy.

Similar Datasets

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