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HepG2 HLA-I immunopeptidome by immunoprecipitation

ABSTRACT: This study utilized mass spectrometry-based proteogenomic profiling to identify the HLA-I immunopeptidome enriched from HepG2 cell. HepG2 WES-based and the COSMIC-based mutation database were generated and utilized to identify HepG2-specific mutant immunopeptides

ORGANISM(S): Homo Sapiens

SUBMITTER: Zhenpeng Zhang

PROVIDER: PXD045203 | iProX | Thu Sep 07 00:00:00 BST 2023

REPOSITORIES: iProX

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COSMIC-based mutation database enhances identification efficiency of HLA-I immunopeptidome.

Wang Fangzhou F Zhang Zhenpeng Z Mao Mingsong M Yang Yudai Y Xu Ping P Lu Shichun S

Journal of translational medicine 20240210 1

<h4>Background</h4>Neoantigens have emerged as a promising area of focus in tumor immunotherapy, with several established strategies aiming to enhance their identification. Human leukocyte antigen class I molecules (HLA-I), which present intracellular immunopeptides to T cells, provide an ideal source for identifying neoantigens. However, solely relying on a mutation database generated through commonly used whole exome sequencing (WES) for the identification of HLA-I immunopeptides, may result i ...[more]

PMID: 38336780

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