Dataset Information

Proteomic analysis of Hypsibius henanensis sp.nov. suffered from 12C6+ heavy ion radiation based on label-free technology

ABSTRACT: Tardigrades are a kind of species that can tolerate ultra-high doses of ionizing radiation. Here, we used heavy ions at different doses of 0,200 and 2000Gy to irradiate Hypsibius henanensis sp.nov.. Label-free proteomic identification was performed on the samples, each dose containing 3 repetitions

ORGANISM(S): Tardigrada

SUBMITTER: Dong Yang

PROVIDER: PXD045537 | iProX | Wed Sep 20 00:00:00 BST 2023

REPOSITORIES: iProX

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Publications

Multi-omics landscape and molecular basis of radiation tolerance in a tardigrade.

Li Lei L Ge Zhengping Z Liu Shihao S Zheng Kun K Li Yaqi Y Chen Kaiqi K Fu Yesheng Y Lei Xiaoguang X Cui Zeling Z Wang Yifan Y Huang Jin J Liu Yanyan Y Duan Mingwang M Sun Zimei Z Chen Jun J Li Liangwei L Shen Pan P Wang Guibin G Chen Junmiao J Li Ruochong R Li Chaoran C Yang Zhixiang Z Ning Yifan Y Luo Arong A Chen Baoyu B Seim Inge I Liu Xin X Wang Fei F Yao Yishan Y Guo Fusheng F Yang Maojun M Liu Cui Hua CH Fan Guangyi G Wang Lizhi L Yang Dong D Zhang Lingqiang L

Science (New York, N.Y.) 20241025 6720

Tardigrades are captivating organisms known for their resilience in extreme environments, including ultra-high-dose radiation, but the underlying mechanisms of this resilience remain largely unknown. Using genome, transcriptome, and proteome analysis of Hypsibius henanensis sp. nov., we explored the molecular basis contributing to radiotolerance in this organism. A putatively horizontally transferred gene, DOPA dioxygenase 1 (DODA1), responds to radiation and confers radioto ...[more]

PMID: 39446960

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Project description:Human deep space and planetary travel is limited by uncertainties regarding the health risks associated with exposure to galactic cosmic radiation (GCR), and in particular the high linear energy transfer (LET), heavy ion component. Here we assessed the impact of two high-LET ions 56Fe and 28Si, and low-LET X rays on genome-wide methylation patterns in human bronchial epithelial cells. We found that all three radiation types induced rapid and stable changes in DNA methylation but at distinct subsets of CpG sites affecting different chromatin compartments. The 56Fe ions induced mostly hypermethylation, and primarily affected sites in open chromatin regions including enhancers, promoters and edges (“shores”) of CpG islands. The 28Si ion-exposure had mixed effects, inducing both hyper and hypomethylation and affecting sites in more repressed heterochromatic environments, whereas X rays induced mostly hypomethylation, primarily at sites in gene bodies and intergenic regions. Significantly, the methylation status of 56Fe ion irradiation sensitive sites, but not those affected by X ray or 28Si ions, could discriminate tumor from normal tissue for human lung adenocarcinomas and squamous cell carcinomas. Thus, high LET radiation exposure leaves a lasting imprint on the epigenome, and affects sites relevant to human lung cancer. The 56Fe ion signature may prove useful in monitoring the cumulative biological impact and associated cancer risks encountered by astronauts in deep space. Genome wide DNA methylation profiling of normal human bronchial epithelial cells irradiated with varying doses of 28Si-ion radiation ( 300 MeV/u at 0, 0.3, 1.0 Gy) , 56Fe-ion radiation (600 MeV/u at 0, 0.1, 0.3, 1.0 Gy) or X rays (320 kV at 0, 1.0 Gy). Triplicate control and irradiated samples were incubated and sampled at 4 timepoints between 2 and 62 days. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles across >485,000 CpGs from collected samples. Samples include: 56Fe ions, 4 doses x 4 time points x 3 replicates (4 removed in QC) = 44 samples; 28Si ions = 3 doses x 4 time points x 3 replicates = 36 samples; X ray, 2 doses x 4 time points x 3 replicates (2 removed in QC)= 22 samples.

Dataset Information

Proteomic analysis of Hypsibius henanensis sp.nov. suffered from 12C6+ heavy ion radiation based on label-free technology

Publications

Multi-omics landscape and molecular basis of radiation tolerance in a tardigrade.

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