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A “Two birds One Stone” Strategy for Site-specific Analysis of the Core Fucosylation and O-GlcNAcylation

ABSTRACT: Core fucosylation and O-GlcNAcylation are two most famous protein glycosylations and regulate diverse physiological and pathological processes in living organism. Core fucosylation is a protein glycosylation in which a L-fucose residue attaches to the innermost N-acetylglucosamine (GlcNAc) of N-glycan through α1, 6 linkage. O-GlcNAcylation is a dy-namic protein glycosylation where D-GlcNAc moieties attaches to serine or threonine residues of proteins. Here, a “two-birds one stone” strategy is described for site-specific analysis of the core fucosylation and O-GlcNAcylation. The de-scribed strategy allows the simultaneously profiling of core-fucosylated glycoproteome and O-GlcNAcylated glycoprote-ome from one complex sample.

ORGANISM(S): Homo Sapiens

SUBMITTER: Hu Zhou

PROVIDER: PXD047486 | iProX | Mon Dec 04 00:00:00 GMT 2023

REPOSITORIES: iProX

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"Two Birds One Stone" Strategy for the Site-Specific Analysis of Core Fucosylation and O-GlcNAcylation.

Luo Yawen Y Wang Yuqiu Y Tian Yinping Y Zhou Hu H Wen Liuqing L

Journal of the American Chemical Society 20230621 29

Core fucosylation and O-GlcNAcylation are the two most famous protein glycosylation modifications that regulate diverse physiological and pathological processes in living organisms. Here, a "two birds one stone" strategy has been described for the site-specific analysis of core fucosylation and O-GlcNAcylation. Taking advantage of two mutant endoglycosidases (EndoF3-D165A and EndoCC-N180H), which efficiently and specifically recognize core fucose and O-GlcNAc, glycopeptides ...[more]

PMID: 37340703

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