Dataset Information

TMT-Based quantitative proteomic analysis reveals the key role of cell proliferation and apoptosis in intestine regeneration of Apostichopus japonicus

ABSTRACT: TMT-Based quantitative proteomic analysis reveals the key role of metabolic process, cell proliferation and apoptosis in intestine regeneration of Apostichopus japonicus

ORGANISM(S): Apostichopus Japonicus

SUBMITTER: Chenghua Li

PROVIDER: PXD050025 | iProX | Thu Feb 22 00:00:00 GMT 2024

REPOSITORIES: iProX

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Publications

TMT-Based Quantitative Proteomic Analysis Reveals the Key Role of Cell Proliferation and Apoptosis in Intestine Regeneration of <i>Apostichopus japonicus</i>.

Zeng Chuili C Xiao Ke K Shi Qilin Q Zhan Xu X Li Chenghua C

International journal of molecular sciences 20240411 8

Sea cucumbers are widely known for their powerful regenerative abilities, which allow them to regenerate a complete digestive tract within a relatively short time following injury or autotomy. Recently, even though the histological changes and cellular events in the processes of intestinal regeneration have been extensively studied, the molecular machinery behind this faculty remains unclear. In this study, tandem mass tag (TMT)-based quantitation was utilized to investigate protein abundance ch ...[more]

PMID: 38673840

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Project description:BackgroundSea cucumbers (Holothuroidea; Echinodermata) have the capacity to regenerate lost tissues and organs. Although the histological and cytological aspects of intestine regeneration have been extensively studied, little is known of the genetic mechanisms involved. There has, however, been a renewed effort to develop a database of Expressed Sequence Tags (ESTs) in Apostichopus japonicus, an economically-important species that occurs in China. This is important for studies on genetic breeding, molecular markers and special physiological phenomena. We have also constructed a library of ESTs obtained from the regenerative body wall and intestine of A. japonicus. The database has increased to ~30000 ESTs.ResultsWe used RNA-Seq to determine gene expression profiles associated with intestinal regeneration in A. japonicus at 3, 7, 14 and 21 days post evisceration (dpe). This was compared to profiles obtained from a normally-functioning intestine. Approximately 5 million (M) reads were sequenced in every library. Over 2400 up-regulated genes (>10%) and over 1000 down-regulated genes (~5%) were observed at 3 and 7dpe (log2Ratio ? 1, FDR ? 0.001). Specific "Go terms" revealed that the DEGs (Differentially Expressed Genes) performed an important function at every regeneration stage. Besides some expected pathways (for example, Ribosome and Spliceosome pathway term), the "Notch signaling pathway," the "ECM-receptor interaction" and the "Cytokine-cytokine receptor interaction" were significantly enriched. We also investigated the expression profiles of developmental genes, ECM-associated genes and Cytoskeletal genes. Twenty of the most important differentially expressed genes (DEGs) were verified by Real-time PCR, which resulted in a trend concordance of almost 100% between the two techniques.ConclusionOur studies demonstrated dynamic changes in global gene expression during intestine regeneration and presented a series of candidate genes and enriched pathways that contribute to intestine regeneration in sea cucumbers. This provides a foundation for future studies on the genetics/molecular mechanisms associated with intestine regeneration.

Dataset Information

TMT-Based quantitative proteomic analysis reveals the key role of cell proliferation and apoptosis in intestine regeneration of Apostichopus japonicus

Publications

TMT-Based Quantitative Proteomic Analysis Reveals the Key Role of Cell Proliferation and Apoptosis in Intestine Regeneration of <i>Apostichopus japonicus</i>.

Similar Datasets

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