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Investigation of the molecular mechanism of Smilax glabra Roxb. in treating hypertension based on proteomics and bioinformatic

ABSTRACT: 100 μg of the peptide mixture of each sample was labeled using TMT reagent, according to the manufacturer’s instructions (Thermo Fisher Scientific). The marked mixed peptide segments were graded using the Agilent 1260 Infinity II HPLC system, and each sample was separated using the EASY-nLC system. After the sample was separated via chromatography, a Q Exactive Plus mass spectrometer was used for mass spectrometry analysis. Twenty fragment maps were collected after each full scan. The isolated peptide segment was analyzed online via Proteome Discoverer 2.4.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Haibing Qian

PROVIDER: PXD051807 | iProX | Sun Apr 28 00:00:00 BST 2024

REPOSITORIES: iProX

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Investigation of the molecular mechanism of <i>Smilax glabra</i> Roxb. in treating hypertension based on proteomics and bioinformatics.

Yang Xin X Qian Haibing H Yang Changfu C Zhang Zhiyuan Z

Frontiers in pharmacology 20240509

<h4>Background</h4><i>Smilax glabra</i> Roxb. (named tufuling in Chinese, SGR) has both medicinal and edible value. SGR has obvious pharmacological activity, especially in anti-inflammation and treating immune system diseases. This study investigated differential protein expression and its relationship with immune infiltration in hypertension treated with SGR using proteomics and bioinformatics.<h4>Methods</h4>N-Nitro L-arginine methyl ester (L-NAME) was used to replicate the hypertension model, ...[more]

PMID: 38783958

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