Investigation of the molecular mechanism of Smilax glabra Roxb. in treating hypertension based on proteomics and bioinformatic

ABSTRACT: 100 μg of the peptide mixture of each sample was labeled using TMT reagent, according to the manufacturer’s instructions (Thermo Fisher Scientific). The marked mixed peptide segments were graded using the Agilent 1260 Infinity II HPLC system, and each sample was separated using the EASY-nLC system. After the sample was separated via chromatography, a Q Exactive Plus mass spectrometer was used for mass spectrometry analysis. Twenty fragment maps were collected after each full scan. The isolated peptide segment was analyzed online via Proteome Discoverer 2.4.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Haibing Qian  

PROVIDER: PXD051807 | iProX | Sun Apr 28 00:00:00 BST 2024

REPOSITORIES: iProX