Dataset Information

High-fat diet alters retinal lipid composition and gene expression networks in mice

ABSTRACT: To reveal whether a long-term HFD alters gene expression levels in the retina, we used 4D-label free mass spectrometry to quantify proteins in the whole retinas of HFM and age-mated NM. As the liver plays a crucial role in metabolism of the whole body, HFD has a huge impact on gene expression in the liver. To compare the similarities and differences in the effects of a HFD on retinal and hepatic gene regulatory networks, we also performed proteomic analyses on the livers from HFM and NM.

ORGANISM(S): Mus Musculus

SUBMITTER: Lin Zhang

PROVIDER: PXD052242 | iProX | Tue May 14 00:00:00 BST 2024

REPOSITORIES: iProX

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High-fat diet alters retinal lipid composition and gene expression networks in mice.

Zou Rong R Cai Jinrui J Chen Tianyu T Mo Wenhui W Qian Hao H Zhu Xianjun X Zhang Lin L

BMC biology 20250417 1

<h4>Background</h4>High-fat diet (HFD) was suggested to be associated with several retinal diseases, including age-related macular degeneration (AMD), glaucoma, and diabetic retinopathy (DR). Nevertheless, our understanding of the mechanisms governing retinal lipid metabolic homeostasis remains limited, with little attention focused on the influence of HFD on different retinal cell types. To address this gap, we established a high-fat model using mice fed with HFD for a duration of 6 months. The ...[more]

PMID: 40247316

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Project description:Obesity and diabetes associated visual impairment and vascular dysfunctions are increasing reasons for vision loss. The detailed mechanisms in these diseases are still largely unknown, but mice models have been useful to study these mechanisms and explore the detailed effects of potential compounds. Such compounds usually have antioxidant and anti-inflammatory properties. These properties are found in anthocyanins and a major source of dietary anthocyanins in Nordic diet is bilberries (European wild blueberries, Vaccinium myrtillus). In this explorative study we show results with differentially expressed genes in retina using a high-fat diet (HFD) mouse model. Our findings displayed differential regulation of genes in pathways for apoptosis, inflammation and oxidative stress, especially systemic lupus erythematosus (SLE), mitogen activated protein kinase (MAPK) and glutathione metabolism. Mice fed with HFD had increased retinal gene expression of several crystallins, which was reduced in the retina of mice fed with bilberries. Bilberries seem to reduce the expression of genes in MAPK and to increase the expression of genes in glutathione metabolism pathway. All together despite minor effects in the mouse phenotype, a diet rich in bilberries may prevent the retinal gene expression changes in the early stages of obesity. Mice were fed ad libitum with normal control diet (NCD, 10% kcal fat), high-fat diet (HFD, 45% kcal fat), 5% (w/w) freeze-dried biberries (Vaccinium myrtillus) in NCD (NCD+BB) or HFD (HFD+BB) for 12 weeks. Diets were prepared in Research Diets Inc. Feed consumption and weight gain were measured during the feeding trial, and blood pressure and serum markers of obesity at the end. Retinas were collected and RNA extracted from all 24 mice samples, and pooled retinas from 4 mice per group were hybridized with standard Illumina protocols. The expression in retinas was analyzed using R, Pathvisio and DAVID to screen for differences between high-fat and berry induced changes to control diets and further bilberry induced changes to HFD up- or downregulated transcripts. diet: NCD, HFD, NCD+BB, HFD+BB

Dataset Information

High-fat diet alters retinal lipid composition and gene expression networks in mice

Publications

High-fat diet alters retinal lipid composition and gene expression networks in mice.

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