Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Fusing Engineered Bacterial Strains with NIR-II AIEgen for Multimodal Image-guided Phototherapy and Immunotherapy

ABSTRACT: we present an aggregation-induced emission (AIE) - bacteria hybrid bionic robot to address the above issues. This bionic robot is composed of a multifunctional AIE molecule, INX-2 and Escherichia coli Nissle 1917 (EcN), i.e. EcN@INX-2. The robot is characterized by NIR-II fluorescence emission with efficient photodynamic and photothermal effects and tumor targeting, which was realized by INX-2 and EcN respectively, EcN@INX-2 achieved in vivo multimodal imaging and therapy of the tumors through multiple mechanisms including the activation of anti-tumor immunity, photodynamic and photothermal therapy in CT26 tumor-bearing female mice.

ORGANISM(S): Mus Musculus

SUBMITTER: Ying Li

PROVIDER: PXD056926 | iProX | Fri Oct 18 00:00:00 BST 2024

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Publications

Aggregation induced emission luminogen bacteria hybrid bionic robot for multimodal phototheranostics and immunotherapy.

Zhu Liwei L Song Guangjie G Zhang Wentian W Wu Yifan Y Chen Yuling Y Song Jiayi J Wang Deliang D Li Guoxin G Tang Ben Zhong BZ Li Ying Y

Nature communications 20250316 1

Multimodal phototheranostics utilizing single molecules offer a "one-and-done" approach, presenting a convenient and effective strategy for cancer therapy. However, therapies based on conventional photosensitizers often suffer from limitations such as a single photosensitizing mechanism, restricted tumor penetration and retention, and the requirement for multiple irradiations, which significantly constrain their application. In this report, we present an aggregation-induced emission luminogen (A ...[more]

PMID: 40089477

Similar Datasets

RNA-Seq-guided triple-combination nano-system of gold nanocluster, quercetin, and docetaxel for ultimate synergistic photothermal-chemotherapy against MDR tumor

Project description:For oncological purposes, hyperthermia has increasingly emerged as a good therapeutic option for patients without fitness to undergo surgery, or for those with surgically inaccessible tumors. However, tumor recurrence after photothermal therapy constitutes a significant barrier to achieving good survival outcome. In this study, we used a gold nanocluster (GNC) micelle system capable of multiple NIR laser exposure; RNA-seq analysis revealed that repeated NIR exposure upregulates heat-shock protein and cell-cycle process-related genes that could be associated with tumor recurrence. To prevent recurrence, we presented an RNA-seq-guided drug combination strategy of GNC, docetaxel, and quercetin via a nano-system. Quercetin treatment downregulated heat-shock protein expression and inhibited function of P-gp with mild antitumor activity. Furthermore, docetaxel treatment demonstrated antitumor activity with significant G2-M cell-cycle arrest. When two of GNC, docetaxel, and quercetin were administered together, they showed a synergistic antitumor effect, whereas when the drugs were administered simultaneously in a triple combination, a very strong synergistic effect was achieved, as indicated through increased apoptosis induction and cell-cycle arrest in vitro and in vivo. The data from this study emphasize the benefit of triple combination and outlines potential translational directions for photothermal-chemotherapy.

2023-02-14 | GSE224908 | GEO

All-in-one Engineering Multifunctional Nanoplatforms for Sensitizing Tumor Low-Temperature Photothermal Therapy in vivo

Project description:To determine the effects of photothermal therapy on regulatory genes and molecular pathways of mouse triple negative breast cancer cells (4T1 cells), we studied the RNA sequences of 4T1 cells treated with medium (control), MoS2 NPs and MoS2 NPs + NIR (808 nm).

2023-10-11 | GSE244922 | GEO

Regulation of undruggable c-MYC with controlled photothermal therapy for efficient cancer immunotherapy

Project description:Cancer immunotherapies have revolutionized the treatment of metastatic cancers however most patients cannot benefit from current immunotherapies due to a suppressive tumor immune microenvironment (TIME). Photothermal therapy (PTT) with nanoparticles (NPs) as photothermal conversion agents has shown potential to induce immunogenic cell death (ICD) by remodeling TIME. PTT with different temperatures have been applied and mild temperature-PTT was recently claimed as the most promising approach. However, the optimal PTT temperature and related mechanisms in activation of immunotherapy have remained unclear. In the present study, black porous silicon (BPSi) NPs coated with polyethylene glycol and cancer cell membranes were utilized as photothermal agents. These NPs have good biocompatibility, high photothermal conversion efficacy, and homotypic tumor targeting ability. It was found that the PTT with the BPSi NPs could efficiently induce ICD, which was largely affected by the applied temperature in PTT. As compared to the mild temperature (46°C) and high temperature (54 °C) PTT, medium temperature PTT at 50 °C was the optimal treatment to induce effective ICD in vitro and inhibit tumor growth in vivo. Furthermore, it was unveiled that PTT could efficiently regulate undruggable c-MYC by tuning temperatures. c-MYC was upregulated by PTT at 46°C while downregulated by PTT at 50 °C. The activities of MYC-induced pathways such as p53 were also significantly enhanced. These findings not only discover the mechanisms in activating immunotherapy with NPs-based PTT but also suggest new directions for developing more potent therapy regimens to treat metastatic tumors.

2025-08-26 | GSE275258 | GEO

All-in-one Engineering Multifunctional Nanoplatforms for Sensitizing Tumor Low-Temperature Photothermal Therapy in vivo

Project description:All-in-one Engineering Multifunctional Nanoplatforms for Sensitizing Tumor Low-Temperature Photothermal Therapy in vivo

| PRJNA1026142 | ENA

Photothermal Modulation of Human Stem Cells Using Light-responsive 2D Nanomaterials

Project description:Two-dimensional (2D) molybdenum disulfide (MoS2) nanomaterials are an emerging class of biomaterials that are photo-responsive at near-infrared wavelengths (NIR). Here, we demonstrate for the first time the ability of 2D MoS2 to modulate cellular functions of human stem cells through photothermal mechanisms. The interaction of MoS2 with human stem cells is investigated using whole transcriptome sequencing (RNA-seq). Global gene expression profile of stem cells reveals significant influence by NIR stimulation of MoS2 on integrins, cellular migration and wound healing. Overall, the combination of MoS2 and NIR light may provide new approach to control and regulate cellular migration and functions.

2020-06-29 | GSE141456 | GEO

Near-infrared light-driven metabolic reprogramming of synoviocytes for the treatment of rheumatoid arthritis

Project description:Rheumatoid arthritis is a common autoimmune disease characterized by chronic synovial inflammation and joint destruction, primarily driven by imbalance cellular metabolism and inflammatory microenvironment. While gene therapy offers a promising therapeutic approach, its effectiveness is limited by the challenges of non-specific gene expression in healthy tissues. In the present study, we developed a gene delivery system (namely APPC), in which near-infrared (NIR)-responsive gold nanorods (AR) were coated with chondroitin sulfate-modified polyethyleneimine (CS-PEI) to facilitate the heat-responsive targeted delivery of heme oxygenase 1 (HO-1) gene. The APPC showed favorable transfection efficiency due to its targeting ability and significantly facilitate the HO-1 expression under NIR irradiation. The combination of APPC/pHO-1 and NIR could effectively reprogram the cellular metabolism and repolarize the macrophages and fibroblast-like synoviocytes (FLSs), thereby inhibiting the inflammation by suppressing the glycolysis. Meanwhile, APPC could specifically enhance the HO-1 expression in inflamed tissues through NIR-mediated the activation of HSP70 promoter, ensuring the precise gene expression via photothermal conversion. In a collagen-induced arthritis (CIA) model, APPC/pHO-1 under NIR irradiation exhibited potent therapeutic efficacy, restoring the articular microenvironmental homeostasis and mitigating the symptoms of RA. These findings highlighted the potential of APPC/pHO-1 nanoparticles in the gene therapy of RA and other inflammatory diseases.

2025-04-17 | GSE294905 | GEO

Engineered bacteria in photothermal hydrogel (EBPH) for metabolic regulating

Project description:We reported the living therapeutic system constructed by the engineered bacteria in photothermal hydrogel (EBPH) for metabolic regulating enhanced tumor immunotherapy (Fig 1). The Escherichia coli strain BL21(DE3) cells were employed and engineered to express lactate oxidase (LOX) and glucose oxidase (GOX) under the control of thermosensitive promoter. The rewired cells were encapsulated into the photothermal hydrogel formed by constructed with sodium alginate (SA) and polypyrrole (PPy). After injection the EBPH around the tumor, the in-situ expression of LOX and GOX was induced by the NIR-II laser irradiation at 1064 nm to catalyze the degradation of glucose and lactate. EBPH could regulate the energy metabolism of tumor cells and relieve the immunosuppressive TME. Meanwhile, EBPH stimulated a series of antitumor immunological responses including the effect of immunogenic cell death (ICD), the activation of cyclic guanosine monophosphate adenosine monophosphate synthase/interferon gene stimulator (cGAS/STING) pathway, the stimulation of T cells, the polarization of macrophages to M1 phenotype and the generation of memory T cells for long term tumor suppression. Our work establishes an approach of living therapeutic hydrogel for metabolic regulating enhanced tumor immunotherapy.

2025-03-28 | PXD062337 |

Janus nanoparticles targeting extracellular polymeric substance achieve flexible elimination of drug-resistant biofilms

Project description:Safe and efficient antibacterial materials are urgently needed to combat drug-resistant bacteria and biofilm-associated infections. The rational design of nanoparticles for flexible elimination of biofilms by alternative strategies remains challenging. Herein, we propose the fabrication of Janus-structured nanoparticles targeting extracellular polymeric substance to achieve dispersion or near-infrared (NIR) light-activated photothermal elimination of drug-resistant biofilms, respectively. Asymmetrical Janus-structured dextran-bismuth selenide (Dex-BSe) nanoparticles are fabricated by a facile strategy to exploit synergistic effects of both components. The biocompatible dextran domain with the maximum exposure endows the Janus nanoparticles with biofilm penetration, targeting, and dispersion functions. Interestingly, Janus Dex-BSe nanoparticles realize enhanced dispersal of biofilms over time compared with dextran nanoparticles while the underlying molecular mechanisms are further revealed by RNA-sequencing transcriptomics analysis. Alternatively, taking advantage of the preferential accumulation of nanoparticles at infection sites, the self-propelled active motion induced by the unique Janus structure enhances the photothermal killing effect under NIR light irradiation, thereby eradicating the biofilm. Given these favorable features, the antibiofilm activity of Janus Dex-BSe against methicillin-resistant Staphylococcus aureus (MRSA) was first validated in vitro. More importantly, the flexible application of Janus Dex-BSe nanoparticles for biofilm removal or NIR-triggered eradication in vivo was demonstrated by MRSA-infected mouse excisional wound model and abscess model, respectively. The currently developed Janus nanoplatform holds great promise for the efficient elimination of drug-resistant biofilms in diverse antibacterial scenarios.

2023-08-01 | GSE239411 | GEO

scRNA-seq reveals tumor microenvironment remodeling induced by local intervention-based immunotherapy

Project description:Laser immunotherapy (LIT) combines local photothermal therapy (PTT), to disrupt tumor homeostasis and release tumor antigens, and an intratumorally administered immunostimulant, N-dihydrogalactochitosan (GC), to induce antitumor immune responses. We performed single-cell RNA sequencing on tumor-infiltrating leukocytes of MMTV-PyMT mouse mammary tumors to determine LIT-induced myeloid and lymphoid compartment remodeling. Analysis of 49,380 single cell transcriptomes from different treatment groups revealed proinflammatory IFNa, IFNg, and TNFa cytokine signaling pathways were enriched in both lymphoid and myeloid cells isolated from LIT-treated tumors. Interestingly, the CD4+ and CD8+ T cells in LIT and GC treated tumors resided in an activated state while immune cells in untreated and PTT-treated tumors remained in a neutral/resting state. Additionally, monocytes recruited into the LIT-treated tumors were driven towards proinflammatory M1-like macrophage phenotypes or monocyte-derived dendritic cells. Our results reveal that LIT prompts immunological remodeling of the tumor microenvironment by initiating broad proinflammatory responses to drive antitumor immunity.

2021-12-29 | GSE150675 | GEO

Chemohormonal Therapy Remodels Prostate Cancer Immune Microenvironment and Enhances Checkpoint Inhibitor-based immunotherapy

Project description:Checkpoint blockade immunotherapy is a promising strategy in cancer treatment, depending on a favorable preexisting tumor immune microenvironment. However, prostate cancer is usually considered as an immune “cold” tumor with the poor immunogenic response and low density of tumor-infiltrating immune cells. This research uses samples from prostate cancer patients showing that docetaxel-based chemohormonal therapy reprograms the immune microenvironment and increases tumor-infiltrating T cells. Mechanistically, docetaxel treatment activates the cGAS/STING pathway and induces the type I interferon signaling, which may boost T cell-mediated immune response. In a murine prostate cancer model, chemohormonal therapy sensitizes tumor-bearing mice to PD1-blockade therapy. These findings demonstrate that docetaxel-based chemohormonal therapy activates prostate cancer immunogenicity and acts cooperatively with anti-PD-1 checkpoint blockade, providing a combination immunotherapy strategy that would lead to better therapeutic benefit for prostate cancer.

2022-08-01 | GSE193898 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data