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Host proteins interacting with EV71-VP2 were identified by mass spectrometry

ABSTRACT: EV71-VP2-MYC was extransfected into 293T cells, and 36 hours later, the cells were treated with MG132 for 12 hours. The cells were harvested, and the cell lysates were incubated with protein-G with MYC antibody overnight. The interacting proteins were analyzed by mass spectrometry.

ORGANISM(S): Homo Sapiens

SUBMITTER: Wenyan Zhang

PROVIDER: PXD059489 | iProX | Tue Jan 07 00:00:00 GMT 2025

REPOSITORIES: iProX

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ZYG11B suppresses multiple enteroviruses by triggering viral VP1 degradation.

Tian Li L Mi Zhizhong Z Yang Weijing W Chen Jing J Wei Xiulong X Zhang Wenyan W Li Zhaolong Z

Journal of virology 20250326 4

Enterovirus 71 (EV71) is a major cause of hand, foot, and mouth disease, particularly affecting pediatric populations worldwide. The role of ZYG11B, a CUL2-complex-associated E3 ubiquitin ligase from the Zyg-11 family, in antiviral defense against EV71 remains unclear. To our knowledge, this study is the first to reveal that ZYG11B targets EV71 VP1 for proteasomal degradation via the ubiquitin-proteasome pathway, with CRL2<sup>ZYG11B</sup> complex activity specifically driving K33-linked ubiquit ...[more]

PMID: 40135890

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Identification of the ubiquitination sites of EV71-VP1

Project description:EV71-VP1-Flag plasmid was transfected into 293T cells for 36 hours and then treated with MG132 for 12 hours. After drug treatment, cells were collected, cell lysates were incubated with FLAG-Beads overnight and competed with FLAG peptides for 4 hours the next day. Proteins were separated by SDS-PAGE and analyzed by mass spectrometry after Coomassie brilliant blue staining.

2025-01-07 | PXD059532 |

Host proteins interacting with EV71-VP1 were identified by mass spectrometry

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