Phosphoproteomic Profiling Reveals PI Pathway and Transcription Factor Regulation in Tamarix hispida under Salt Stress

ABSTRACT: Here, we identify the salt-sensitive proteins in vivo under 200 mM NaCl treatment in Taramix hispida by 4D label free-based phosphoproteomic analysis. Through a normalization by proteomics analysis to phosphorylation omics, 5,425 phosphorylated sites were identified in 1,960 comparable proteins. Meanwhile, 132 proteins were screened as differential expression phosphorylated proteins (DEPPs) including 94 upregulated phosphoproteins containing 159 phosphorylated sites and 38 downregulated phosphoproteins containing 50 phosphorylated sites. The DEPPs were enriched in various pathways such as photosynthesis, nuclear localization signals, and signal transduction process. The upregulated phosphorylation of key enzymes in PI-PLC signal transduction process like PIP5K and DGK enhanced salt stress tolerance of T.hispida. What’s more, we also identified 52 transcription factors that might respond salt stress conditions through phosphorylation. Five of them were positively regulated salt stress tolerance of T.hispida by phosphorylation or dephosphorylation. More important, we also suggest a scheme depicting the molecular events that NaCl stress activates the PI signaling pathway in T.hispida. These findings provide a landscape of salt-sensitive proteins of Taramix in response to salt stress, which provide even more significant sheds new light for facilitate research on the breeding of salt-tolerant plant varieties.

ORGANISM(S): Tamarix Hispida

SUBMITTER: Yucheng Wang  

PROVIDER: PXD064704 | iProX | Fri Jun 06 00:00:00 BST 2025

REPOSITORIES: iProX