Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparative proteomic analysis of outer membrane vesicles from Vibrio parahaemolyticus and highly lethal Vibrio parahaemolyticus

ABSTRACT: Acute hepatopancreatic necrosis disease (AHPND) is a major shrimp disease caused by specific strains of Vibrio parahaemolyticus (VpAHPND). More recently, a newly emerged outbreak known as translucent post-larvae disease (TPD), caused by highly lethal Vibrio parahaemolyticus (HLV), has severely impacted the shrimp aquaculture industry due to its extremely high mortality in Litopenaeus vannamei. Outer membrane vesicles (OMVs), nanoscale extracellular structures secreted by vibrios, play crucial roles in virulence factor delivery and host-pathogen interactions. In this study, we conducted a systematic proteomic comparison of OMVs derived from VpAHPND and HLV. The results showed that both types of OMVs exhibited typical features of Gram-negative bacteria and shared a large number of conserved proteins associated with outer membrane maintenance, nutrient transport, and basic metabolic processes, suggesting common roles in fundamental physiology and host interactions. However, notable differences were also observed. Specifically, compared with VpAHPND-OMVs, HLV-OMVs were enriched with proteins related to toxins, secretion systems, immune modulation, and metabolic adaptation. These findings suggest that HLV-OMVs may possess enhanced capabilities to promote host infection, evade immune defenses, and adapt to environmental stress. Overall, this study not only reveals the shared characteristics of VpAHPND-OMVs and HLV-OMVs but also highlights the unique protein composition of HLV, providing an important proteomic resource for elucidating the pathogenic mechanisms of V. parahaemolyticus from the perspective of OMVs.

ORGANISM(S): Vibrio Parahaemolyticus Vp2007-007

SUBMITTER: Mengqiang Wang

PROVIDER: PXD068354 | iProX | Sun Sep 14 00:00:00 BST 2025

REPOSITORIES: iProX

ACCESS DATA

Json Xml

Similar Datasets

Shrimp transcriptome analysis after exposure to recombinant Vibrio parahaemolyticus PirA and PirB toxins

Project description:Vibrio parahaemolyticus is a Gram-negative bacterium commonly found in marine and estuarine environments. Acute hepatopancreatic necrosis disease (AHPND) caused by this bacterium is an ongoing problem among shrimp farming industries. V. parahaemolyticus proteins PirA and PirB have been determined to be major virulence factors that induce AHPND. In this study, Pacific white shrimp (Litopenaeus vannamei) were challenged with recombinant PirA and PirB by a reverse gavage method and then at 30 m, 1, 2, 4, and 6 h time points, the hepatopancreas of five individual shrimp were removed and placed into RNA later. We conducted RNA sequencing of the hepatopancreas samples from a no PirA/B control (n = 5) and PirA/B-treated shrimp at the different time intervals (n=5). We evaluated the different gene expression patterns between the time groups to the control with a focus on identifying differences in innate immune function.

2023-05-05 | GSE200137 | GEO

OpaR Controls a Network of Downstream Transcription Factors in Vibrio parahaemolyticus BB22

Project description:Vibrio parahaemolyticus an emerging pathogen that is a causative agent of foodborne gastroenteritis when raw or undercooked seafood is consumed. Previous microarray data using a Vibrio parahaemolyticus RIMD2210633 chip has shown the master quorum-sensing regulator OpaR controls virulence, type III and type VI secretion systems, and flagellar and capsule production genes. In a parallel study, RNA-Seq was used to comparatively study the transcriptome changes of wild type Vibrio parahaemolyticus BB22 and a ΔopaR strain directly. Differences in mRNA expression were analyzed using next generation Illumina sequencing and bioinformatics techniques to align and count reads. A comparison with the previous microarray data showed good correlation between the shared genes. The RNA-Seq offered an insight into control of genes specific to the Vibrio parahaemolyticus BB22 strain as well as a new look at the sRNAs that are expressed. Eleven transcriptional regulators with greater than 4 fold regulation in the previous microarray study and 2 fold regulation in the RNA-Seq analysis, were chosen to validate the data using qRT-PCR and further characterized with electrophoretic mobility shift assays (EMSAs) to determine if they are direct targets of OpaR. The transcription factors chosen play key roles in virulence, surface motility, cell to cell interactions, and cell surface characteristics. One small RNA was identified in the RNA-Seq data to be quorum-sensing controlled and unidentified by other programs. The RNA-Seq data has aided in understanding and elucidating the hierarchy of quorum-sensing control of OpaR in Vibrio parahaemolyticus. The wild type Vibrio parahaemolyticus BB22 strain LM5312 and an opaR deletion strain LM5674 were analyzed for mRNA expression using RNA-Seq.

2013-12-31 | E-GEOD-53639 | biostudies-arrayexpress

OpaR Controls a Network of Downstream Transcription Factors in Vibrio parahaemolyticus BB22

2013-12-31 | GSE53639 | GEO

Vibrio parahaemolyticus RIMD 2210633 T6SS2 secretome

Project description:Comparative proteomics to identify proteins found in the media of Vibrio parahaemolyticus RIMD 2210633 bacteria with an active T6SS2 compared to bacteria with inactive T6SS2. Bacteria with an active T6SS2 are Vibrio parahaemolyticus RIMD 2210633 inwhich hcp1 was deleted to inactivate T6SS1. T6SS2 inactive bacteria are the former strain with an additional deletion in hcp2. Both strains express TfoX from an arabinose-inducible plasmid to induce T6SS2 activity.

2023-02-05 | PXD037980 | Pride

Vibrio parahaemolyticus BB22OP T6SS2 secretome

Project description:Compare the secreted proteins of a wild-type Vibrio parahaemolyticus strain with those of a mutant in hcp2, rendering the T6SS2 inactive

2023-02-05 | PXD037864 | Pride

Transcriptome analysis of pacific white shrimp (Litopenaeus vannamei) hepatopancreas in response to Vibrio parahaemolyticus inoculation

Project description:In order to gain a better understanding of the impact of Vibrio parahaemolyticus infection on genetic regulation of Litopenaeus vannamei,we performed a transcriptome analysis in the hepatopancreas of Litopenaeus vannamei challenged with Vibrio parahaemolyticus, using the Illumina HiSeq 2500 platform.

2020-06-18 | GSE107697 | GEO

Vibrio parahaemolyticus gene expression affected by 0.04% bile

Project description:Vibrio parahaemolyticus is a Gram-negative marine bacterium. Strain RIMD 2210633, the wild type strain of the organism, causes acute gastroenteritis in humans. Human intestinal factor bile often affects the global gene regulation in some species of enteropathogenic bacteria. To determine the genes in V. parahaemolyticus that respond to bile, we investigated the differences in the transcriptomes of the wild type strain and the vtrA-null strain grown in Luria-Bertani medium cultivated with or without 0.04% crude bile. The vtrA gene encodes the previously identified T3SS2 regulator. Our goal is to demonstrate bile regulon controlled by VtrA in V. parahaemolyticus.

2010-10-10 | GSE21666 | GEO

RNA sequencing analysis of the genes regulated by CueR in Vibrio parahaemolyticus.

Project description:The transcriptome of the wild type strain and ΔcueR of Vibrio parahaemolyticus was compared by RNA sequencing analysis.

2025-06-14 | GSE299205 | GEO

Transcriptomic analysis of Vibrio parahaemolyticus interaction with phage VpP2326.

Project description:The transcriptome changes of Vibrio parahaemolyticus in normal culture and pahge VpP2326 infection were studied by RNA sequencing. The data showed that 39 genes were up-regulated and 2 genes were down-regulated.

2025-06-10 | GSE270444 | GEO