Proximity labeling-based proteomic analysis of the HEV ORF1-associated replication complex under EIF4H deficiency

ABSTRACT: This project explores the role of EIF4H in regulating the composition of the HEV ORF1-associated replication complex using proximity-dependent biotin labeling with TurboID. TurboID was inserted into a validated permissive site within the ORF1 coding region that allows insertion without disrupting ORF1 function. HEK293T wild-type and EIF4H-knockout cells expressing ORF1-TurboID were subjected to biotin labeling, streptavidin-based enrichment, and mass spectrometry analysis. Comparative proteomics identified EIF4H-dependent host factors associated with the HEV replication complex.

ORGANISM(S): Homo Sapiens

SUBMITTER: Qiang Ding  

PROVIDER: PXD069279 | iProX | Thu Oct 09 00:00:00 BST 2025

REPOSITORIES: iProX