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Proteomic Profiling of an ALS Drosophila Model Reveals the Impact of Scaffold Degrader P3 on Head Tissue

ABSTRACT: This project presents a quantitative proteomic analysis of Drosophila melanogaster head tissue expressing human FUS-P525L, using data-independent acquisition (DIA) mass spectrometry. To enable identification of the human transgene, mass spectrometry data were analyzed against a custom database containing the Drosophila melanogaster proteome (UniProt UP000000803) and the human FUS sequence. Head samples were processed through protein extraction, acetone precipitation, and in-solution tryptic digestion. LC-MS/MS data were acquired on a Thermo Scientific instrument and analyzed with the DIA-NN software (v1.8.1), with FDR controlled at 1%. The dataset enables the investigation of protein expression changes, including the transgenic human FUS, under P3 treatment.

ORGANISM(S): Drosophila Melanogaster

SUBMITTER: JiaHeng Tan

PROVIDER: PXD069579 | iProX | Fri Oct 17 00:00:00 BST 2025

REPOSITORIES: iProX

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