Project description:Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control Embryos were exposed to 10uM TMT or control from 48hpf to 60 or 72 hpf. Three replicates were collected for each time point. 40 embryos were pooled to comprise a replicate.
Project description:Investigation of whole genome expression pattern of 60 and 72 hours post fertilization Danio Rerio embryos exposed to TMT and vehicle control
Project description:This experiment is a TMT10plex labelled experiment containing trypsin digested UPS1 and UPS2 human proteins in replicate along with E.Coli lysates. The mass spectra were generated in a single MS2-based experiment, using TMT10plex-labelled samples made up of UPS1, UPS2, and E.Coli lysate. UPS1 (Universal Proteomics Standard) and UPS2 (Proteomics Dynamic Range Standard) are publicly available reference standards, used in mass spectrometry (supplied by Sigma-Aldrich) [33]. Both consist of the same 48 recombinant human proteins (ranging from 6,000Da to 83,000Da in mass), present in equal abundance in UPS1 and at different abundances in UPS2 (from 500 amoles to 50 pmoles).
Project description:This dataset contains quantitative tandem mass tag (TMT)-based proteomic profiling of mouse oocytes across defined experimental conditions. 500, fully grown, prophase I-arrested oocytes were isolated from young adult females and matured in vitro to metaphase II eggs.
Project description:to assess the role of neutrophil-produced collagen in skin injury. 3 mouse groups (WT, Mrp8-Cre;TGFbR2-floxed and Mrp8-Cre;iDTR) were subjected to an ear injury by piercing. A limited area around the wound was dissected (6-10 mg tissue) and subjected to ECM extraction and TMT proteomics analysis. From each animal, an equivalent amount of skin from the other (healthy) ear was also excised and used as an internal control. 4 biological replicates per condition (WT, A and B) with 2 samples per animal (injured and healthy control) were distributed in three independent TMT 10-plex experiments. Samples from the same animal were kept together in the same TMT experiment. In each TMT experiment one of the channels was reserved for a common internal standard (I.S.) made by pooling the 24 peptide samples.
Project description:We study the repertoire of immunoglobulin sequences after immunization in a cohort of rats. Animals were immunized with dinitrophenol modified KLH or with recombinant human HuD. In the immune reprtoire, and also in the matching proteomics data, we observe that the animals produce a repertoire that has many shared motifs for those immunized with the same antigen. Cluster analysis allows the samples to be segregated according to the immunogen used.