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FLAG-IP–MS profiling of SPINDOC interactome using a HeLa–Tet-On–FLAG-SPINDOC-GFP cell line

ABSTRACT: HeLa–Tet-On–FLAG-SPINDOC-GFP cells were established for inducible expression and affinity purification. FLAG-SPINDOC-GFP expression was first induced by doxycycline treatment. Subsequently, cells were either left unsynchronized or arrested at the G2/M phase by nocodazole treatment. Efficient expression and enrichment of FLAG-SPINDOC-GFP were confirmed by FLAG co-immunoprecipitation. Immunoprecipitates were then subjected to mass spectrometry analysis to identify SPINDOC-associated proteins, particularly under mitotic conditions.

ORGANISM(S): Homo Sapiens

SUBMITTER: Guohong Li

PROVIDER: PXD078443 | iProX | Fri May 15 00:00:00 BST 2026

REPOSITORIES: iProX

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