DARTS-MS chemoproteomic profiling of ginkgolide J-associated protease-susceptibility candidate proteins in rat PASMC lysates

ABSTRACT: This dataset contains DIA-MS raw files and processed DIA-NN results from a DARTS-MS chemoproteomic experiment designed to identify ginkgolide J-associated protease-susceptibility candidate proteins in primary rat pulmonary artery smooth muscle cell (PASMC) lysates. The experiment included three groups with three biological replicates per group: DMSO vehicle control, protease-only group, and ginkgolide J plus protease group. PASMC protein lysates in the ginkgolide J plus protease group were incubated with ginkgolide J at 25 μM before limited proteolysis with Pronase E. DARTS-MS was used as a target-discovery chemoproteomic approach to detect ligand-associated changes in protease susceptibility rather than conventional steady-state protein expression changes.

ORGANISM(S): Rattus Norvegicus

SUBMITTER: Xiangyun Gai  

PROVIDER: PXD078800 | iProX | Mon May 25 00:00:00 BST 2026

REPOSITORIES: iProX