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HSD17B4 interactome in mouse keratinocytes reveals association with splicing factors and RNA processing

ABSTRACT: This project aims to elucidate the molecular mechanism by which HSD17B4 regulates CYP7B1 expression in mouse keratinocytes (mKCs). To identify proteins that interact with HSD17B4, we performed co-immunoprecipitation (Co-IP) using an HSD17B4-specific antibody, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The raw mass spectrometry data were acquired and processed to identify HSD17B4-interacting proteins. Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the HSD17B4 interactome revealed a significant overrepresentation of proteins involved in RNA splicing and RNA processing. These findings suggest that HSD17B4 may regulate CYP7B1 expression through interactions with splicing factors, pointing to a previously unrecognized nuclear function of HSD17B4 beyond its enzymatic activity. This project contains the MS data for the interacting protein of HSD17B4 we identified.

ORGANISM(S): Mus Musculus

SUBMITTER: Honglin Wang

PROVIDER: PXD081170 | iProX | Thu Jul 16 00:00:00 BST 2026

REPOSITORIES: iProX

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