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Intestinal β-galactosidase affects host health by regulating flavonoid metabolism

ABSTRACT: Untargeted metabolomic analysis was performed to identify the principal aglycone generated by the engineered Escherichia coli Nissle 1917 strain expressing β-galactosidase (EcN-bgal). Culture supernatants from EcN-bgal and the vector control strain were collected and subjected to LC–MS-based metabolomic profiling. Comparative analysis revealed substantial alterations in the metabolite composition of the EcN-bgal supernatant, enabling the identification of β-galactosidase-dependent metabolites and the characterization of the major aglycone produced by the engineered strain.

ORGANISM(S): Escherichia Coli Nissle 1917

SUBMITTER: Shenhai Gong

PROVIDER: PXD082253 | iProX | Wed Aug 05 00:00:00 GMT+01:00 2026

REPOSITORIES: iProX

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