Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Spermatophylax of Poecilimon ampliatus liquid sample

ABSTRACT: The spermatophore was removed and separated into ampulla and spermatophylax. Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. The supernatant was analyzed directly by LC-FTICR MS.

ORGANISM(S): Poecilimon Ampliatus

SUBMITTER: Peter Jungblut

PROVIDER: PXD005027 | JPOST Repository | Sat Sep 23 00:00:00 BST 2017

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	140614_Lehmann_Phylanx.mzXML	Mzxml
	LTQ_140614_Lehmann_Phylanx_liquid.mgf	Mgf
	Phylax_liquid.xml	Xml

Items per page:

1 - 3 of 3

Similar Datasets

Ampulla of Poecilimon ampliatus liquid sample

2017-09-23 | PXD005029 | JPOST Repository

Ampulla of Poecilimon amplicata 2-DE spots 9,10,11

Project description:The spermatophore was removed and separated into ampulla and spermatophylax. Each of these compartments was solubilized in 6M urea, 3 M thiourea, 70 mM DTT and centrifuged. The supernatant was separated by 2-DE and spots 9,10, and 11 were analyzed by LC-FTICR MS. Spots 9,10, and 11 were occuring on 2-DE patterns of both spermatophylax and ampulla.

2017-09-23 | PXD005030 | JPOST Repository

Ampulla of Poecilimon ampliatus

Project description:Nuptial gifts of P.ampliatus were divided into ampullae and spermatophylax. Both were separated by high-resolution 2-DE with a gel size of 23 cm x 30 cm and stained with FireSilver. 3 spots of the spermatophylax were excised, trypsin digested and analyzed by LC-FTICR.

2017-09-22 | PXD005024 | JPOST Repository

Spermatophylax of Poecilimon ampliatus

Project description:Nuptial gifts of P. ampliatus were divided into ampullae and spermatophylax. Both were separated by high-resolution 2-DE with a gel size of 23 cm x 30 cm and stained with FireSilver. 16 spots of the spermatophylax were excised, trypsin digested and analyzed by LC-FTICR.

2017-09-22 | PXD005023 | JPOST Repository

Plasma Sample

Project description:Plasma Cell-free DNA Sequencing data with human reads removed

| PRJNA865093 | ENA

rumen liquid sample

Project description:rumen liquid sample Metagenome

| PRJNA1466632 | ENA

Gryllodes sigillatus isolate:Spermatophylax

Project description:Gryllodes sigillatus spermatophylax cDNA

| PRJNA291561 | ENA

Sample preparation for proteomics

Project description:Urea-containing buffer solutions are generally used in proteomic studies to aid protein denaturation and solubilisation during cell and tissue lysis. It is well-known, however, that urea can lead to the carbamylation of peptides and proteins and; subsequently, incomplete digestion of proteins. In our work, we sought for ways to retain urea in the digestion protocol to reduce sample handling, while minimizing urea-related drawbacks.

2018-06-06 | PXD009426 | Pride

Fallopian tube vs Fimbria

Project description:Compare luteal and follicular phases of fimbria and ampulla tissue Fallopian tube epithelial cells for gene expression analysis of fimbria and ampulla specimens were obtained using LCM

2020-04-04 | GSE129348 | GEO

Removed

Project description:Removed

| PRJEB28844 | ENA