Proteomics

Dataset Information

0

DIA proteomics of dried blood spot proteins by sodium carbonate

ABSTRACT: We developed a simple and sensitive analysis method for dried blood spot (DBS) proteins by sodium carbonate precipitation (SCP). We aimed to removing large amounts of hydrophilic blood proteins such as hemoglobin, albumin, fibrinogen and transferrin in DBS using a simple pretreatment with sodium carbonate solution, which concentrates membrane proteins. We evaluated the performance of the SCP method by comparing the LC-MS/MS results obtained for proteins prepared by SCP with those obtained by sodium dodecanate (SoDod) extract as total protein solubilized from DBS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Daisuke Nakajima 

PROVIDER: PXD017185 | JPOST Repository | Mon Jan 20 00:00:00 GMT 2020

REPOSITORIES: jPOST

Json Xml

Dataset's files

Source:
Action DRS
SCP_5GPFs.sdia Other
SCP_DBS.raw Raw
SCP_DBS_GPF1.raw Raw
SCP_DBS_GPF2.raw Raw
SCP_DBS_GPF3.raw Raw
Items per page:
1 - 5 of 11
altmetric image

Publications

Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics.

Nakajima Daisuke D   Kawashima Yusuke Y   Shibata Hirofumi H   Yasumi Takahiro T   Isa Masahiko M   Izawa Kazushi K   Nishikomori Ryuta R   Heike Toshio T   Ohara Osamu O  

Journal of proteome research 20200604 7

Dried blood spots (DBS) are widely used for screening biomolecular profiles, including enzymatic activities. However, detection of minor proteins in DBS by liquid chromatography-mass spectrometry (LC-MS/MS) without pre-enrichment remains challenging because of the coexistence of large quantities of hydrophilic proteins. In this study, we address this problem by developing a simple method using sodium carbonate precipitation (SCP). SCP enriches hydrophobic proteins from DBS, allowing substantial  ...[more]

Similar Datasets

Transcriptomics Transcriptome profiles derived from parallel venipuncture, dried blood spot (DBS), and saliva samples
2016-07-27 | GSE79269 | GEO
Proteomics High Field Asymmetric Waveform Ion Mobility Spectrometry in Non-targeted Bottom-up Proteomics of Dried Blood Spot
2018-05-03 | PXD007926 | Pride
Proteomics Evaluation of suitability of dried saliva spots for in-depth proteome analyses of clinical applications
2022-04-26 | PXD031181 | JPOST Repository
Transcriptomics Peripheral blood transcriptome profiles derived from parallel venipuncture (PAXgene and mononuclear cell; PBMC) and dried blood spot (DBS) samples.
2016-05-18 | GSE75511 | GEO
Clinical Integrating Hepatitis C Screening With Dried Blood Spot Testing Into Colorectal Cancer Screening
| 2312242 | ecrin-mdr-crc
Proteomics Evaluation of suitability of dried saliva spots for in-depth proteome analyses of clinical applications - Additional file
2022-04-26 | PXD032116 | JPOST Repository
Proteomics Development of non-targeted proteome analysis of dried blood spots using an automated protein extraction system
2025-12-12 | PXD058804 | JPOST Repository
Proteomics Development of non-targeted proteome analysis of dried blood spots using an automated protein extraction system (
2025-08-14 | PXD064701 | JPOST Repository
Transcriptomics Transcriptome profiles derived from parallel venipuncture, dried blood spot (DBS), and saliva samples
2016-07-27 | E-GEOD-79269 | biostudies-arrayexpress
Genomics MicroRNA sequencing of BIBINS HIE dried blood spot
2021-08-05 | GSE181127 | GEO