Proteomics

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DIA proteomics of dried blood spot proteins by sodium carbonate

ABSTRACT: We developed a simple and sensitive analysis method for dried blood spot (DBS) proteins by sodium carbonate precipitation (SCP). We aimed to removing large amounts of hydrophilic blood proteins such as hemoglobin, albumin, fibrinogen and transferrin in DBS using a simple pretreatment with sodium carbonate solution, which concentrates membrane proteins. We evaluated the performance of the SCP method by comparing the LC-MS/MS results obtained for proteins prepared by SCP with those obtained by sodium dodecanate (SoDod) extract as total protein solubilized from DBS.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Daisuke Nakajima 

PROVIDER: PXD017185 | JPOST Repository | Mon Jan 20 00:00:00 GMT 2020

REPOSITORIES: jPOST

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Publications

Simple and Sensitive Analysis for Dried Blood Spot Proteins by Sodium Carbonate Precipitation for Clinical Proteomics.

Nakajima Daisuke D   Kawashima Yusuke Y   Shibata Hirofumi H   Yasumi Takahiro T   Isa Masahiko M   Izawa Kazushi K   Nishikomori Ryuta R   Heike Toshio T   Ohara Osamu O  

Journal of proteome research 20200604 7

Dried blood spots (DBS) are widely used for screening biomolecular profiles, including enzymatic activities. However, detection of minor proteins in DBS by liquid chromatography-mass spectrometry (LC-MS/MS) without pre-enrichment remains challenging because of the coexistence of large quantities of hydrophilic proteins. In this study, we address this problem by developing a simple method using sodium carbonate precipitation (SCP). SCP enriches hydrophobic proteins from DBS, allowing substantial  ...[more]