Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Leaf of amphibious plant developed under terrestrial or submerged condition

ABSTRACT: Proteomics analysis of amphibious plant leaves. Leaves developed under terrestrial or submerged condition were used.

ORGANISM(S): Cellular Organisms

SUBMITTER: Genki Horiguchi

PROVIDER: PXD024779 | JPOST Repository | Tue Jun 15 00:00:00 BST 2021

REPOSITORIES: jPOST

ACCESS DATA

Json Xml

Publications

Transition From Proto-Kranz-Type Photosynthesis to HCO3 - Use Photosynthesis in the Amphibious Plant Hygrophila polysperma.

Horiguchi Genki G Matsumoto Kaori K Nemoto Kyosuke K Inokuchi Mayu M Hirotsu Naoki N

Frontiers in plant science 20210616

Hygrophila polysperma is a heterophyllous amphibious plant. The growth of H. polysperma in submerged conditions is challenging due to the low CO2 environment, increased resistance to gas diffusion, and bicarbonate ion (HCO3 -) being the dominant dissolved inorganic carbon source. The submerged leaves of H. polysperma have significantly higher rates of underwater photosynthesis compared with the terrestrial leaves. 4,4'-Diisothiocyanatostilben ...[more]

PMID: 34220895

Similar Datasets

Gene expression of Arabidopsis leaves under heat stress and during recovery

Project description:To understand plant adaptation to heat stress, gene expression profiles of Arabidopsis leaves under heat stress, during recovery and control condition were obtained using microarray. Microarray data listed responsible candidate genes for glycerolipid metabolism.

2015-05-27 | GSE63128 | GEO

Expression data in an Arabidopsis cytokinin (CK)-signaling mutant, ahp2,3,5 and its wild type plant (Col-0) under drought and well-water (control) conditions.

Project description:To understand the role of CK-signaling components, AHPs (His-containing phosphotransfer proteins) in drought stress response, we have employed transcriptional profiling of ahp2,3,5 and it's wild type plant, Col-0 under drought and well-water (control) conditions. AligentM-bM-^@M-^Ys Whole Arabidopsis Gene Expression Microarray (G2519F-021169, V4, 4x44K) was used. Two-week-old WT and ahp2,3,5 mutant plants were transferred from GM plates to soil and grown for an additional week. Three week-old plants were exposed to drought stress for 10 days or grown under well-watered condition in parallel. All rosette leaves of independent 31d-old plants were corrected. Total RNA was prepared and used for the microarray hybridization. Three replicative hybridization experiments were carried out for each independent biological sample.

2013-02-15 | E-GEOD-42290 | biostudies-arrayexpress

Microarray analysis of gene expression under different light conditions

Project description:The size of tomato fruits are largely dependent on growth conditions. To obtain insights on how light intensity contributes to translocation from a leaf and a fruit, we developed a plant irradiation system based on light-emitting diodes (LEDs) to a leaf. By using this system, we investigated the changes of transcript profiles of tomato leaves and fruits grown under different light conditions.

2012-05-02 | GSE35020 | GEO

Gene expression profile of rice shoot and root in response to nitrogen deficiency treatment under phosphorus deficiency condition.

Project description:Nitrogen (N) and phosphorus (P) are pivotal element for proper plant growth and development. We performed microarray analysis of rice shoot and root after nitrogen deficiency (-N) treatment under phosphorus deficiency (-P) condition to obtain a global view of gene regulations associated with plant response to -N under -P condition.

2019-10-06 | GSE73775 | GEO

Comparative Phosphoproteome Analysis of Seedling Leaves in Brachypodium distachyon. under Different Drought Conditions

Project description:Drought stress is a major abiotic stress affecting plant growth and development. Brachypodiumdistachyon L. is a special model plant for wheat, barley, and several potential biofuel grasses. In this study, we make comparative phosphoproteome analysis of seeding leaves at the three leaf stage in Brachypodiumdistachyon L. for the different treatment conditions (6h, 24h). A total of 480 unique phosphopeptides, representing 439 phosphoproteins, were identified under drought stress. Motif-X analysis identified six motifs, such as [SP], [RxxS], [LxRxxS], [SxD], [SF] and [TP]. Molecular function and PPI analysis suggested that many proteins may take participation in signal transduction, gene expression, drought response and defense with energy metabolism and material transmembrane transport under moisture deficiency condition. Further analyses revealed that these SCPL phosphoproteins may play important roles in regulating signal transduction and material transmembrane transport to cope with the adverse environmental conditions. This study first focus on the effects of phosphorylation modification mechanisms on the growth and development of seeding leaves at different drought stresses.

2018-10-26 | PXD003522 | Pride

Phosphoproteomics reveals regulation of secondary metabolites in Mahonia bealei under ultraviolet-B radiation

Project description:Mahonia Bealei was used as a traditional Chinese medicine for its high alkaloid content. Previous research found that alkaloid and flavonoid contents in the M. bealei leaves increased under combinatory treatments of ultraviolet B and dark. In order to explore the underlying response mechanism, TiO2 material enrichment and mass-based label-free quantitative proteomics techniques were used for phosphoproteomics analysis of M. bealei leaves under ultraviolet B. ATP content, photosynthetic pigment content, and some enzymatic/non-enzymatic indicators increased in the leaves of M. bealei under UV-B radiation. Phosphoproteomics study found that under the UV-B radiation, phosphoproteins related to MAPK signal transduction and plant hormone brassinosteroid signaling pathway were varied greatly. Phosphoproteins related to photosynthesis, glycolysis, tricarboxylic acid cycle, and amino acid synthesis/metabolism pathway were also significantly changed. These results suggested that the ultraviolet B radiation activated oxidative stress system, MAPK signal transduction pathway, and photosynthetic energy metabolism pathway. These changes are important for the redox reactions in secondary metabolism and the accumulation of secondary metabolites in M. bealei leaves under UV-B radiation.

2022-02-16 | PXD028873 | Pride

Gene expression profile of leaf in rice grown under paddy field with macronutrient deficiencies

Project description:Macronutrients are pivotal elements for proper plant growth and development. We performed microarray analysis of rice leaves under nitrogen (N), phosphorus (P), and potassium (K) deficiency conditions in paddy field to obtain a global view of gene regulations associated with plant response to essential nutrients.

2019-10-06 | GSE73766 | GEO

Microarray analysis of gene expression under different light conditions

2012-05-01 | E-GEOD-35020 | biostudies-arrayexpress

Real-time quantitative PCR analysis of cucumber mitogen activated protein kinase cascade genes under various stress conditions and plant hormone treatments

Project description:We used Bio-Rad laboratories, lnc. PCR assay panel to analyze expression levels of MAPK genes under various stress conditions and plant hormone treatments. Cucumber plants of the ‘Jinyan No.4’ cultivar were reared in growth chambers at 28 ± 1 °C with a photoperiod of 16 h light/8 h dark and light intensity of 400 μmol m−2 s−1. Three-week-old seedlings were used for all abiotic and biotic treatments. For heat or cold treatment, the seedlings were subjected to 35 ± 1 °C or 4 ± 1 °C conditions, respectively. The samples for RNA extraction were collected at 0, 1, 2, 4, and 8 h after treatment. For dehydration treatment, the leaves of plants were sampled at 0, 2, 4, and 6 d after watering stopped. For disease treatment, Pseudoperonospora cubensis (P.cubensis) was used to infect the seedlings, and leaves were collected at 0, 1, 2, and 3 d after infection. For hormone treatments, the seedling leaves were sprayed with 100 mM methyl jasmonate (meJA) or 100 mM abscisic acid (ABA) and then sampled at 0, 1, 2, 4, and 8 h intervals.

2015-05-01 | GSE68436 | GEO

Internal reference gene selection under salt, alkali, and drought stresses in ecological remediation plant giant reed

Project description:Real-time quantitative PCR (RT–qPCR) is the favoured method for gene expression analysis in molecular biology due to its sensitivity, specificity, cost-effectiveness, and reproducibility. To obtain the accuracy and reliability of RT-qPCR, the use of reliable reference genes is inevitable. There were many reports about the physiological response of giant reed (Arundo donax L.) to abiotic stresses. However, there is little use in the validation of reference genes under different treatments. It still belongs to the blank that the research about selecting reference genes under salt and alkali. In this study, the expression stability of twenty-three candidate reference genes in leaves and roots were assessed under salt, drought, and alkali stresses using geNorm, NormFinder, BestKeeper, and Delta Ct algorithms. Our results showed that no one gene had an invariant expression under different conditions. For example, under drought stress, UPL3, UBC2, and APT1 were better reference genes in leaves, RPL5 and FPS2 were better in roots. Under alkali stress, GAPDH, APT1, and RPS5 were better reference genes in leaves; UPL3, ACT2, and SAMDC2 were better in roots. In addition, the expression of MSD1 was used to further confirm the validated reference genes under salt, drought, and alkali stresses. It was proved that the use of inappropriate reference genes in giant reed significantly altered the relative expression of target genes and even reversed the results. Consequently, our results provided guidelines for reference gene selection under salt, drought, and alkali stresses and a foundation for more accurate and widespread use of RT-qPCR in the giant reed.

2021-12-15 | GSE190890 | GEO