Project description:Smoke released from burning vegetation functions as an important environmental signal promoting the germination of many plant species following a fire . It not only promotes the germination of species from fire-prone habitats, but several species from non-fire-prone areas also respond, including some crops. Bioactivity-guided fractionation of smoke-water led to the identification of a highly active butenolide compound, 3-methyl-2H-furo[2,3-c]pyran-2-one. Several hypotheses have arisen regarding the molecular background of smoke and butenolide action. Contrary to the efforts to unravel the mode of action of smoke, the mechanism is still largely unknown. In this paper we demonstrate that although smoke-water and butenolide treatment of maize kernels results in a similar physiological response, the gene and protein expression patterns are quite different. Treatment with smoke-water enhanced the ubiquitination of proteins and activated protein-degradation-related genes. This effect was completely absent from butenolide-treated kernels, in which a specific aquaporin gene was distinctly upregulated. These findings indicate that other bioactive compounds present in smoke-water may act together, leading to accelerated protein turnover. The results highlight the importance of protein degradation and aquaporins in the seed germination process. Besides their obvious use in the sustainable agricultural practice, smoke and butenolide can be used in studies to gain further insight into the transcriptional changes during germination.
Project description:Purpose: To compare RNASeq data of Frankia CcI3 in plants under salt stress. Casuarina glauca root nodules infected with Frankia CcI3 were exposed to either no salt or 100 mM NaCl for 21 days. RNA-seq analysis provided insight into how the sybiont responds to salt stress.
Project description:Plant pathogens require lethal virulence factors, susceptible hosts, and optimal environmental conditions for disease establishment. High soil salinity, exacerbated by climate change, significantly impacts agro-biological ecosystems. However, the overall interactions between plant pathogens and salt stress are not fully characterized or understood. This study examines the effects of salt stress on representative plant pathogens: Burkholderia gladioli, Pectobacterium carotovorum subsp. carotovorum, and Ralstonia solanacearum. Using pan-genome-based comparative transcriptomics, we analyzed the comprehensive alterations within the biological systems of plant pathogens when treated with 200 mM NaCl. Our results highlight the differential responses between salt-sensitive and salt-tolerant pathogens to salt stress.
Project description:Arundo donax L. is one of the most promising bioenergy crop due to its high biomass yield and low irrigation requirement. The resistance to biotic and abiotic stress causes the high invasiveness of this plant which can grow with very low management input (e.g., pesticides, fertilization, irrigation) even in marginal lands or in fields irrigated with waste or salty water. Despite its economic importance, the A. donax genomic resources are still limited. In particular, no information on its transcriptional response to salt stress is available.We report the leaf transcriptome sequencing, de novo assembly and annotation of a giant reed genotype under two levels of salt stress. The study will be useful for providing insight into the molecular mechanism underlying its extreme adaptability also offering a platform for directing future efforts towards the genetic improvement of this species.
Project description:GR24, a synthetic strigolactone, and KAR1, the main bioactive compound in smoke water, both share a common α,β unsaturated furanone moiety which promotes biomass accumulation in three week old N. benthamiana seedlings. In order to investigate whether this D ring is responsible for the biomass accumulation, gene expression profiles were evaluated for co-expression on the Agilent 44k N. tabacum microarray. GR24, smoke and KAR1 induced different transcripts, and suggests that they trigger independent growth responses.
Project description:Salt stress is one of the major abiotic stresses affecting the yield of ginseng (Panax ginseng C. A. Meyer). The objective of this study was to identify proteins of ginseng, which is responsive in salt stress. In this direction, ginseng plants of different growth stages (3, 4 and 5 years), were grown in the hydroponic conditions and exposed to 5 ds/m salt concentration. The secreted proteins, collected from the water, at 0, 24, 72 and 120 hours after exposure were used for the proteome analysis using shotgun approaches. Through the shotgun proteomics, a total of 155 and 88 secreted proteins were identified by searching in two RNA-sequencing (RNA-seq) database, respectively.
Project description:GR24, a synthetic strigolactone, and KAR1, the main bioactive compound in smoke water, both share a common α,β unsaturated furanone moiety which promotes biomass accumulation in three week old N. benthamiana seedlings. In order to investigate whether this D ring is responsible for the biomass accumulation, gene expression profiles were evaluated for co-expression on the Agilent 44k N. tabacum microarray. GR24, smoke and KAR1 induced different transcripts, and suggests that they trigger independent growth responses. Control (untreated), GR24 (10-7M), smoke water (1:1000 dilution) and butenolide (10-7M) gene expression profiles were evaluated on three week old seedlings, in two independent experimental trials.
Project description:To understand the molecular mechanisms of Suaeda salsa under salt stress, RNA-seq analysis was used to identify genes expressed in Suaeda salsa during salt stress response.