Proteomics

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Crosslinking mass spectrometry analysis of enriched spindle pole body sample

ABSTRACT: Crosslinking mass spectrometry (CLMS) analysis was conducted on the enriched Saccharomyces cerevisiae spindle pole body (SPB) preparation to explore the structure of the SPB, particularly focusing on its topology and the interactions between subunits.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Juri Rappsilber 

PROVIDER: PXD050440 | JPOST Repository | Mon Nov 18 00:00:00 GMT 2024

REPOSITORIES: jPOST

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B240110_10_Astral_ZC_IN_101_BS3xSPB_pepSECf7.raw Raw
B240110_11_Astral_ZC_IN_101_BS3xSPB_pepSECf8.raw Raw
B240110_12_Astral_ZC_IN_101_BS3xSPB_pepSECf9.raw Raw
B240110_14_Astral_ZC_IN_101_BS3xSPB_pepSECf10.raw Raw
B240110_15_Astral_ZC_IN_101_BS3xSPB_pepSECf11.raw Raw
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Publications

Structure of the native γ-tubulin ring complex capping spindle microtubules.

Dendooven Tom T   Yatskevich Stanislau S   Burt Alister A   Chen Zhuo A ZA   Bellini Dom D   Rappsilber Juri J   Kilmartin John V JV   Barford David D  

Nature structural & molecular biology 20240412 7

Microtubule (MT) filaments, composed of α/β-tubulin dimers, are fundamental to cellular architecture, function and organismal development. They are nucleated from MT organizing centers by the evolutionarily conserved γ-tubulin ring complex (γTuRC). However, the molecular mechanism of nucleation remains elusive. Here we used cryo-electron tomography to determine the structure of the native γTuRC capping the minus end of a MT in the context of enriched budding yeast spindles. In our structure, γTu  ...[more]

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