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Time-resolved analysis of vUNG phosphorylation

ABSTRACT: Time-resolved phosphoproteomic analysis of HEp-2 cells infected with wild type HSV-1(F)

ORGANISM(S): Cellular Organisms

SUBMITTER: Masaaki Oyama

PROVIDER: PXD055199 | JPOST Repository | Tue Jun 03 00:00:00 BST 2025

REPOSITORIES: jPOST

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			Action	DRS
	20231004_hata_20231004_kato_36h.xlsx	Xlsx
	20231004_kato_36h_FAIMS_05.raw	Raw
	20231017_hata_20231017_kato_24h.xlsx	Xlsx
	20231017_kato_24h_FAIMS_05.raw	Raw
	20231018_hata_20231018_kato_12h.xlsx	Xlsx

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1 - 5 of 12

Publications

Herpes simplex virus 1 evades APOBEC1-mediated immunity via its uracil-DNA glycosylase in mice.

Kato Akihisa A Harima Hayato H Tsunekawa Yuji Y Igarashi Manabu M Kitamura Kouichi K Wakae Kousho K Nishiyama Tomoaki T Morimoto Satoru S Suzuki Toru T Kozuka-Hata Hiroko H Oyama Masaaki M Motooka Daisuke D Watanabe Mizuki M Takeshima Kousuke K Maruzuru Yuhei Y Koyanagi Naoto N Okano Hideyuki H Inada Toshifumi T Okada Takashi T Muramatsu Masamichi M Kawaguchi Yasushi Y

Nature microbiology 20250603

Herpes simplex virus 1 (HSV-1) is the most common cause of viral encephalitis, which can be lethal or result in severe neurological defects despite antiviral therapy. The apolipoprotein B messenger-RNA editing enzyme, catalytic polypeptide-like (APOBEC) group of proteins can act as viral restriction factors. How HSV-1 evades this intrinsic immune mechanism is unclear. Here, using human carcinoma HEp-2 cells, we find that phosphorylation and therefore activation of HSV-1 uracil-DNA glycosylase co ...[more]

PMID: 40461648

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Time-resolved phosphoproteomic analysis of ZIKV-infected cells

Project description:To investigate cellular pathway dysregulation upon ZIKV infection, time-resolved phosphoproteomics LC-MS/MS analysis was used. ZIKV-infected SK-N-BE2 cells or mock-treated cells were harvested 24, 48 and 72 hours post virus infection and their phosphoproteome was quantitatively analyzed by label-free LC-MS/MS.

2018-09-03 | PXD009560 | Pride

MS-based determination of vUNG phosphorylation

Project description:Phosphoproteomic analysis of HEp-2 cells infected with wild type HSV-1(F)

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Time-resolved genome-wide pathogenic gene expression analysis of the plant pathogen Xanthomonas oryzae pv. oryzae (Xoo) via RNA-Seq

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Time-resolved genome-wide pathogenic gene expression analysis of the plant pathogen Xanthomonas oryzae pv. oryzae (Xoo) via RNA-Seq

Project description:Host-pathogen interactions, in particular during the early stages of infection, are important for the fate of both the invading pathogen and host. In pathogens, these interactions activate diverse pathogenic signals to cause disease in the host and evade the inevitably activated immune responses of the infected host. In this study, we used in vitro assay system and RNA-seq to analyze the time-resolved genome-wide gene expressions of Xanthomonas oryzae pv. oryzae (Xoo) evoked by the interactions with rice leaf extracts. RNA-seq analysis was carried out with samples of 7 different time points within 1 h. The RNA-seq data were verified by qRT-PCR. The in vitro system successfully initiated the pathogenic signals in Xoo cell culture. Many pathogenicity-related genes were expressed within 15 min. The hrpG gene was transcribed at maximum level within 10 min, and hrpX gene expression reached maximum level in 15 min. Chemotaxis and flagellar biosynthesis-related genes and cyclic-di-GMP synthesizing genes having GGDEF motif, were down-regulated until 10 min and after then up-regulated. Genes related to iron uptake and two component systems of phoB-phoR, phoP-phoQ, and pmrA-pmrB were up-regulated before 5 min. Data of multiple time points enabled non-linear regression fit of the time-resolved expression data, with which we newly developed the continuous time-resolved heat map to help the comparison of gene expression profiles. Essentiality of the transcriptionally up-regulated genes was also analyzed by the pathogenicity assay using the Xoo knockout strains, which showed not necessarily all the up-regulated genes were required for pathogenicity. the time-dependent, 0, 5, 10, 15, 30, 45, and 60 min, transcriptome analysis of genome-wide Xanthomonas oryzae pv. oryzae genes from the pathogenic interactions with RLX via RNA-Seq

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