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Chitooligosaccharide and Epigallocatechin Gallate Conjugation Improves Metabolic Dysfunction and Adipose Tissue Inflammation in High Fat Diet Induced Obese Rats

ABSTRACT: Obesity has progressively become a global concern. It contributes to adipose tissue dysfunction, leading to metabolic disorders, and chronic systemic inflammation. Our previous study showed that the conjugation between chitooligosaccharides (COS) and epigallocatechin-3-gallate (EGCG) diminished lipid accumulation and promoted browning of white adipose tissue (WAT) in high fat diet (HFD)-induced obese rats.

ORGANISM(S): Cellular Organisms

SUBMITTER: Chainarong Tocharus

PROVIDER: PXD062110 | JPOST Repository | Sat Mar 21 00:00:00 GMT 2026

REPOSITORIES: jPOST

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	AT-1_1.mzXML	Mzxml
	AT-1_2.mzXML	Mzxml
	AT-1_3.mzXML	Mzxml
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	AT-2_2.mzXML	Mzxml

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Administration of hydro-alcoholic extract of spinach improves oxidative stress and inflammation in high-fat diet-induced NAFLD rats.

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| S-EPMC8418034 | biostudies-literature

An Isocaloric High-Fat Diet Improves Lifespan in Rats and Drosophila

Project description:The health effect of dietary fat has been one of the most vexing issues in the nutrition field. Few animal studies have examined the impact of high-fat diets on lifespan by controlling energy intake. In this study, we found that compared to a normal diet, an isocaloric high-fat diet (IHF) significantly prolonged lifespan by decreasing the profiles of free fatty acids (FFAs) in serum and multiple tissues by downregulating FFAs anabolism and upregulating catabolism pathways in rats and flies. Proteomics analysis in rats identified PPRC1 as a key protein that was significantly upregulated by nearly 2-fold by IHF, and among the FFAs, only palmitic acid (PA) was robustly and negatively associated with the expression of PPRC1. Using PPRC1 transgenic RNAi/overexpression flies and in vitro experiments, we further demonstrated that IHF significantly reduced PA, which could upregulate PPRC1 through PPARG, resulting in improvements in oxidative stress and inflammation and prolonging lifespan.

2021-01-06 | PXD022585 | Pride

Adipocyte small extracellular vesicle-derived microRNA-30a-3p exacerbates hepatic steatosis in high fat diet-fed male mice

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Gene expression profiles reveal the effect of a high-fat diet in brown fat development

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Crocetin improves the insulin resistance induced by high-fat diet in rats.

Project description:BACKGROUND AND PURPOSE: The amelioration of insulin resistance by treatment with crocetin is closely related to the hypolipidaemic effect. The present study is designed to clarify the insulin-sensitizing mechanism of crocetin by elucidating the mechanism of regulation of lipid metabolism by crocetin. EXPERIMENTAL APPROACH: Rats given a high-fat diet were treated with crocetin for 6 weeks before hyperinsulinaemic-euglycaemic clamp. 14C-palmitate was used as tracer to track the fate of non-esterified fatty acids or as substrate to measure beta-oxidation rate. Triglyceride clearance in plasma and lipoprotein lipase activity in tissues were tested. Content of lipids in plasma and tissues was determined. Real-time PCR was used to assay the level of mRNA from genes involved in non-esterified fatty acid and triglyceride uptake and oxidation. KEY RESULTS: Crocetin prevented high-fat-diet induced insulin resistance (increased clamp glucose infusion rate), raised hepatic non-esterified fatty acid uptake and oxidation, accelerated triglyceride clearance in plasma, enhanced lipoprotein lipase activity in liver, and reduced the accumulation of detrimental lipids (DAG and long-chain acyl CoA) in liver and muscle. Genes involved in hepatic lipid metabolism which are regulated by peroxisome proliferator-activated receptor-alpha, were modulated to accelerate lipid uptake and oxidation. CONCLUSIONS AND IMPLICATIONS: Through regulating genes involved in lipid metabolism, crocetin accelerated hepatic uptake and oxidation of non-esterified fatty acid and triglyceride, and reduced lipid availability to muscle, thus decreasing lipid accumulation in muscle and liver, and consequently improving sensitivity to insulin.

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<i>Gynura procumbens</i> ethanol extract improves vascular dysfunction by suppressing inflammation in postmenopausal rats fed a high-fat diet.

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