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Nuclear proteome analysis of mouse preimplantation embryos

ABSTRACT: Mouse embryos at the 2-cell to 8-cell stages were collected and treated with acidic Tyrodeâ€™s solution to remove the zona pellucida. Subsequently, embryos were split into single blastomeres, and nuclei were isolated from each blastomere using a micromanipulator. Proteomic samples were then prepared using the in situ digestion of alcohol-fixed cells (iSDAC) method with 50 (2-cell), 216 (4-cell), and 280 (8-cell) nuclei per sample. The nuclear proteome during mouse preimplantation development was identified by label-free quantification.

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Masaki Matsumoto

PROVIDER: PXD063271 | JPOST Repository | Fri Apr 24 00:00:00 BST 2026

REPOSITORIES: jPOST

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			Action	DRS
	20220623_EVO40SPD_FAIMS45_15uDIA_2Cnuc_01.raw	Raw
	20220623_EVO40SPD_FAIMS45_15uDIA_2Cnuc_02.raw	Raw
	20220623_EVO40SPD_FAIMS45_15uDIA_4Cnuc_01.raw	Raw
	20220623_EVO40SPD_FAIMS45_15uDIA_4Cnuc_02.raw	Raw
	20220623_EVO40SPD_FAIMS45_15uDIA_8Cnuc_01.raw	Raw

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