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Label-free vacuolar proteomics of wild-type and vps35Δ yeast using Orbitrap Eclipse

ABSTRACT: Vacuoles were isolated from wild-type and vps35Δ S. cerevisiae cells grown to stationary phase. Peptides were digested using Preomics iST kit, analyzed by Thermo Orbitrap Eclipse in DDA mode with FAIMS (-45V/-65V). Data were searched against UniProt yeast database using Proteome Discoverer 2.4.1.15 with 10 ppm precursor and 0.02 Da fragment tolerances, filtered at 1% FDR. This dataset was used to identify retromer cargoes accumulating in vps35Δ vacuoles.

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Prof. Jieqiong Gao

PROVIDER: PXD080926 | JPOST Repository | Mon Jul 13 00:00:00 BST 2026

REPOSITORIES: jPOST

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			Action	DRS
	BY4741-vps35%20vs%20wt.xlsx	Xlsx
	BY4741-vps35.raw	Raw
	BY4741-vps35.xlsx	Xlsx
	BY4741-wt.raw	Raw
	BY4741-wt.xlsx	Xlsx

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