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Fungal Induced Protein Hyperacetylation Identified by Acetylome Profiling

ABSTRACT: Here we investigate how the effector molecule HC-toxin, a histone deacetylase inhibitor, produced by Cochliobolus carbonum promotes pathogen virulence in maize through altering protein acetylation. Using mass spectrometry we globally quantified the abundance of 3,636 proteins and 2,791 acetylation sites in maize plants treated with HC-toxin as well as HC-toxin deficient or producing strains of C. carbonum. Analysis of these data demonstrates that acetylation is a widespread post-translational modification impacting proteins encoded by many intensively studied maize genes. Furthermore, the application of exogenous HC-toxin enabled us to show that the activity of plant-encoded enzymes can be modulated to alter acetylation of non-histone proteins during an immune response. Collectively, these results provide a resource for further mechanistic studies examining the regulation of protein function and offer insight into the complex immune response triggered by virulent C. carbonum.

INSTRUMENT(S): LTQ Velos

ORGANISM(S): Zea Mays (ncbitaxon:4577)

SUBMITTER: Justin W Walley

PROVIDER: MSV000079681 | MassIVE | Wed Apr 27 09:00:00 BST 2016

REPOSITORIES: MassIVE

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