Proteomics

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Proteomic analysis of glutamine deprivation in HepG2 cells

ABSTRACT: We applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. Two independent cultured cell lysates, namely control and glutamine deprivation, were marked with iTRAQ labels and subjected to LC-ESI-MS/MS analysis. The tryptic peptides of two samples were labeled with mass 113 (control) and 119 (glutamine deprivation) isobaric iTRAQ tags.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Xianghua Yan  

PROVIDER: MSV000080721 | MassIVE | Wed Mar 29 00:59:00 BST 2017

SECONDARY ACCESSION(S): PXD003387

REPOSITORIES: MassIVE

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Publications

Effects of different fertilization treatments on soil microbial functional diversity of dry tableland wheat field in south Shanxi Province.

Liu Lian-Lian LL   Shang Yan-Meng YM   Zhang Jie J   Li Ting-Liang TL   Xie Ying-He YH   Xie Jun-Yu JY   Li-Na L I LI   Hong Jian-Ping JP  

Ying yong sheng tai xue bao = The journal of applied ecology 20230301 3

Understanding the effects of different fertilization treatments on microbial functional diversity in loess tableland wheat soil in south Shanxi Province can provide the theoretical basis from the perspective of microbial functional diversity for chemical fertilizer reduction, wheat yield increase, and soil fertility improvement in dryland soil. We conducted a long-term field experiment with seven fertilization treatments in winter wheat cultivation area of loess tableland in south Shanxi Provinc  ...[more]