Proteomics

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Proteomic analysis of glutamine deprivation in HepG2 cells

ABSTRACT: We applied the quantitative proteomics approach to estimate the relative abundance of proteins in HepG2 cells treated by glutamine deprivation. Two independent cultured cell lysates, namely control and glutamine deprivation, were marked with iTRAQ labels and subjected to LC-ESI-MS/MS analysis. The tryptic peptides of two samples were labeled with mass 113 (control) and 119 (glutamine deprivation) isobaric iTRAQ tags.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Xianghua Yan  

PROVIDER: MSV000080721 | MassIVE | Wed Mar 29 00:59:00 BST 2017

SECONDARY ACCESSION(S): PXD003387

REPOSITORIES: MassIVE

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Publications

Effect of a carbohydrṇate lollipop on the gastric volume of fasted pediatric patients.

Odendaal Pieter P   Burke Annemie A   Coetzee Johan J  

Paediatric anaesthesia 20220520 9

<h4>Background</h4>Preoperative fasting is part of routine practice. Children subjected to prolonged preoperative fasting often suffer adverse effects. Consuming a preoperative lollipop may lessen their anxiety and have clinical benefits.<h4>Aims</h4>To assess the effect of consuming a lollipop on gastric volume and the feasibility of administering a lollipop to a child preoperatively.<h4>Methods</h4>In this prospective, repeated measures interventional study, we measured gastric antrum volume u  ...[more]