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Identification and spectral annotation of the threespine stickleback gill proteome

ABSTRACT: Gill proteome samples from four populations of threespined sticklebacks; Six biological replicates each for 4 different populations: Bodega Harbor, CA (fully plated, marine), Lake Solano (low-plated, freshwater), Laguna de la Bocana del Rosario, Mexico (low plated, brackish water, warm-adapted), Westchester Lagoon, AK (fully plated, brackish water); More information and results of data analysis at CAMP Proteome, accession number CAMPDDA00023 (https://kueltzlab.ucdavis.edu/CAMP_dda_profiles.cfm?AC=CAMPDDA00023).

INSTRUMENT(S): impact

ORGANISM(S): Gasterosteus Aculeatus (ncbitaxon:69293)

SUBMITTER: Dietmar Kueltz

PROVIDER: MSV000081795 | MassIVE | Sat Dec 09 19:21:00 GMT 2017

SECONDARY ACCESSION(S): PXD008395

REPOSITORIES: MassIVE

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Development of a Gill Assay Library for Ecological Proteomics of Threespine Sticklebacks (<i>Gasterosteus aculeatus</i>).

Li Johnathon J Levitan Bryn B Gomez-Jimenez Silvia S Kültz Dietmar D

Molecular & cellular proteomics : MCP 20180809 11

A data-independent acquisition (DIA) assay library for quantitative analyses of proteome dynamics has been developed for gills of threespine sticklebacks (<i>Gasterosteus aculeatus</i>). A raw spectral library was generated by data-dependent acquisition (DDA) and annotation of tryptic peptides to MSMS spectra and protein database identifiers. The assay library was constructed from the raw spectral library by removal of low-quality, ambiguous, and low-signal peptides. Only unique proteins represe ...[more]

PMID: 30093419

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