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High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics

ABSTRACT: In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and performed in 96-well format. We combine this technology with in situ hepatic stimulation, to generate time-resolved maps of insulin signaling in the mouse liver.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Matthias Mann

PROVIDER: MSV000082502 | MassIVE | Mon Jun 25 11:41:00 BST 2018

SECONDARY ACCESSION(S): PXD001792

REPOSITORIES: MassIVE

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Publications

High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics.

Humphrey Sean J SJ Azimifar S Babak SB Mann Matthias M

Nature biotechnology 20150817 9

Mass spectrometry has enabled the study of cellular signaling on a systems-wide scale, through the quantification of post-translational modifications, such as protein phosphorylation. Here we describe EasyPhos, a scalable phosphoproteomics platform that now allows rapid quantification of hundreds of phosphoproteomes in diverse cells and tissues at a depth of >10,000 sites. We apply this technology to generate time-resolved maps of insulin signaling in the mouse liver. Our results reveal that ins ...[more]

PMID: 26280412

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High-throughput phosphoproteomics reveals in vivo insulin signaling dynamics

Project description:In this project we report 133 biologically distinct mouse liver cell (Hepa 1-6 and FL83B) and mouse tissue (brain, kidney, and liver) phosphoproteomes prepared using a newly developed scalable, single-run phosphoproteomics platform, using titanium dioxide (TiO2) based phosphopeptide enrichment and performed in 96-well format. We combine this technology with in situ hepatic stimulation, to generate time-resolved maps of insulin signaling in the mouse liver.

2015-08-19 | PXD001792 | Pride

Gene expression profile of 3D liver organoids composed of hepatocyte and Kupffer cells exposed to nanoparticles.

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Titanium dioxide nanoparticles exposure

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2010-12-22 | E-GEOD-17902 | biostudies-arrayexpress

iTRAQ-based Quantitative Phosphoproteomics Revealed Differences in Phosphorylation Modification Between Different Toxoplasma gondii Genotypes

Project description:iTRAQ technology combine with titanium dioxide (TiO2) affinity chromatography was applied to investigate the phosphoproteomic difference between type I (RH) strain, type II Prugniuad (PRU) strain and Chinese 1 (PYS) strain genotypic tachyzoites of T. gondii

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Titanium dioxide nanoparticles exposure

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An Adaptive Response through N6-Methyladenosine in Fetal Offspring following Gestational Nano-TiO2 Inhalation Exposure II

Project description:Background: N6-methyladenosine (m6A) is the most prominent epitranscriptomic modification to RNA in eukaryotes, but it’s role in adaptive changes within the gestational environment are poorly understood. Nano titanium dioxide (TiO2) exposure is common during pregnancy, though the impact fetal progeny is not entirely understood. We propose that gestational exposure to nano-TiO2 contributes to cardiac m6A methylation in fetal offspring and indirectly contributes to mitochondrial dysfunction.

2022-08-24 | GSE211480 | GEO

An Adaptive Response through N6-Methyladenosine in Fetal Offspring following Gestational Nano-TiO2 Inhalation Exposure I

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RNA sequencing analysis shows that titanium dioxide nanoparticles induce endoplasmic reticulum stress, which has a central role in mediating plasma glucose in mice

Project description:Titanium dioxide nanoparticles (TiO2 NPs) constitute the top five NPs in use today. A recent study determined that oral administration of TiO2 NPs increases plasma glucose in mice. In the current study, RNA sequencing (RNA-seq) technology was used to investigate genome-wide effects of TiO2 NPs. Clustering analysis of the RNA-seq data showed the most significantly enriched gene ontology terms and KEGG pathways related to the endoplasmic reticulum (ER) and ER stress. Molecular biology verification showed that TiO2 NPs activated a xenobiotic biodegradation response and increased expression of cytochrome P450 family genes in mouse livers, thus inducing ER stress in mice. ER stress activated MAPK and NF-B pathways and induced an inflammation response, resulting in phosphorylation of the insulin receptor substrate 1 and, consequently, insulin resistance. This was the main mechanism by which TiO2 NPs increased plasma glucose in mice. Meanwhile, ER stress disturbed the monooxygenase system, and thus generated reactive oxygen species (ROS). Relief of ER stress with 4-phenylbutyric acid inhibited all the above effects of TiO2 NPs, including the generation of ROS. Therefore, TiO2 NP-induced ER stress was a decisive factor with a central role in plasma glucose disturbance in mice.

2019-11-14 | GSE107084 | GEO

Effect of Titanium dioxide nanoparticles on Escherichia coli K12 MG1655 genes expression

Project description:In the present study, we investigated the transcriptional expression patterns of the model strain E. coli exposed to titanium dioxide nanoparticles (NP-TiO2), under dark conditions by using a microarray. Expression profiles were compared to unexposed E.coli and ratio of expression were analysed. Cell exposure to NP-TiO2 was conducted in 10 mM NaCl, E. coli bacterial suspension and NP-TiO2 stock suspension (or mQ water for the control) were added to the NaCl solution to obtain final concentrations of 10E7 cells/ml and 100 mg/l of TiO2 nanoparticles. The flasks were then incubated at 20M-BM-0C on a dark rotary shaker for 5 h. Exposed NP-TiO2: 4 biological replicats. Control: 4 biological replicats.

2014-09-08 | E-GEOD-55972 | biostudies-arrayexpress

Diurnal regulatory landscape of the mouse liver nucleus

Project description:To dissect how diurnal rhythms affect key functions such as transcription or chromatin remodelling, we quantified the temporal nuclear accumulation of proteins and phosphoproteins from mouse liver using in vivo stable isotope labelling by amino acids in cell culture (SILAC)-based mass spectrometry (MS). Protein extracts from isotope labelled mice liver nuclei were used as a reference and mixed with extracts from animals collected every 3 h for 45 h total. Phosphopeptide levels were analysed after enrichment with titanium dioxide (TiO2). The proteins levels were also analysed (dataset with project accession PXD003818).

2016-12-22 | PXD004191 | Pride

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