Dataset Information

ITRAQ-based Quantitative Phosphoproteomics Revealed Differences in Phosphorylation Modification Between Different Toxoplasma gondii Genotypes

ABSTRACT: iTRAQ technology combine with titanium dioxide (TiO2) affinity chromatography was applied to investigate the phosphoproteomic difference between type I (RH) strain, type II Prugniuad (PRU) strain and Chinese 1 (PYS) strain genotypic tachyzoites of T. gondii

INSTRUMENT(S): Q Exactive

ORGANISM(S): Toxoplasma Gondii

TISSUE(S): Whole Body

DISEASE(S): Disease Free

SUBMITTER: Ze-Xiang Wang

LAB HEAD: XingQuan Zhu

PROVIDER: PXD007777 | Pride | 2019-11-14

REPOSITORIES: Pride

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Publications

iTRAQ-Based Global Phosphoproteomics Reveals Novel Molecular Differences Between Toxoplasma gondii Strains of Different Genotypes.

Wang Ze-Xiang ZX Zhou Chun-Xue CX Calderón-Mantilla Guillermo G Petsalaki Evangelia E He Jun-Jun JJ Song Hai-Yang HY Elsheikha Hany M HM Zhu Xing-Quan XQ

Frontiers in cellular and infection microbiology 20190823

To gain insights into differences in the virulence among T. gondii strains at the post-translational level, we conducted a quantitative analysis of the phosphoproteome profile of T. gondii strains belonging to three different genotypes. Phosphopeptides from three strains, type I (RH strain), type II (PRU strain) and ToxoDB#9 (PYS strain), were enriched by titanium dioxide (TiO2) affinity chromatography and quantified using iTRAQ technology. A total of 1,441 phosphopeptides, 1,250 p ...[more]

PMID: 31508380

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Project description:Phosphorylation is a regulatory mechanism by which intracellular apicomplexan parasites control the process of invading and modifying host cells. However, the sporulated oocysts’ phosphoprotein repertoires between virulent and avirulent strains of Toxoplasma gondii and the underlying mechanisms contributing to virulence is still a mystery. A quantitative analysis of the sporulated oocysts’ phosphoproteome profile of T. gondii virulent and avirulent strains belonging to different genotypes was conducted to reveal phosphoprotein expression patterns that contribute to the virulence of a particular phenotype. Phosphopeptides from the sporulated oocysts of the virulent strain PYS (Chinese ToxoDB#9) and the avirulent strain type II (PRU strain) were enriched by titanium dioxide (TiO2) affinity chromatography and quantified using iBT technology. A total of 10,645 unique phosphopeptides, 8,181 nonredundant phosphorylation sites and 2,792 phosphoproteins were identified. The quantitative analysis identified 4,129 differentially expressed phosphopeptides (DEPs) between sporulated oocysts of the PYS strain and PRU strain (|log1.5 fold change| > 1 and P < 0.05), including 2,485 upregulated and 1,644 downregulated phosphopeptides. Motif analysis identified 24 motifs from the upregulated phosphorylated peptides including 22 serine motifs and two threonine motifs (TPE and TP), and 15 motifs from the downregulated phosphorylated peptides including 12 serine motifs and three threonine motifs (TP, RxxT and KxxT) in PYS strain when comparing PYS/PRU. Several kinases were consistent with motifs of overrepresented phosphopeptides, such as PKA, PKG, CKII, IKK, MAPK, EGFR, INSR, Jak, Syk, Src, Ab1. GO analysis, KEGG pathway analysis and STRING analysis revealed differentially expressed phosphoproteins (DEPs) exhibiting significant functional properties. Kinase associated network analysis showed that AGC kinase had the greatest connected peptides. Our findings reveal significant difference in phosphopeptides of sporulated oocysts between virulent and avirulent T. gondii strains, which provides solid foundation for further exploring the mechanisms contributing to the virulence of T. gondii.

Dataset Information

ITRAQ-based Quantitative Phosphoproteomics Revealed Differences in Phosphorylation Modification Between Different Toxoplasma gondii Genotypes

Publications

iTRAQ-Based Global Phosphoproteomics Reveals Novel Molecular Differences Between <i>Toxoplasma gondii</i> Strains of Different Genotypes.

Similar Datasets

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