Proteomics

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TAILS N-terminomics of the human dental pulp stroma and the odontoblast layer


ABSTRACT: We report the targeted analysis of the human dental pulp stroma and the odontoblast layer using the positional proteomics technique TAILS N-terminomics, which allowed us to capture both naturally blocked and unblocked protein N-termini, and to identify differences between e.g. the dental pulp proteomes of older and younger donors. Noteworthy, these differences were most pronounced in the odontoblast region. Note: This study is a follow-up on PXD002264.

INSTRUMENT(S): 6520 Quadrupole Time-of-Flight LC/MS

ORGANISM(S): Homo sapiens  

SUBMITTER: Prof. Christopher Mark Overall  

PROVIDER: MSV000083951 | Massive | Mon Jun 10 14:08:00 BST 2019

SECONDARY ACCESSION(S): PXD006557

REPOSITORIES: MassIVE

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Publications

The Human Odontoblast Cell Layer and Dental Pulp Proteomes and N-Terminomes.

Abbey S R SR   Eckhard U U   Solis N N   Marino G G   Matthew I I   Overall C M CM  

Journal of dental research 20171016 3


The proteome and N-terminome of the human odontoblast cell layer were identified for the first time by shotgun proteomic and terminal amine isotopic labeling of substrates (TAILS) N-terminomic analyses, respectively, and compared with that of human dental pulp stroma from 26 third molar teeth. After reverse-phase liquid chromatography-tandem mass spectrometry, >170,000 spectra from the shotgun and TAILS analyses were matched by 4 search engines to 4,888 and 12,063 peptides in the odontoblast cel  ...[more]

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