Project description:3 samples of R1, R2 and R3 bone marrow monocytes were compared from 3 biological replicates in 3 separate experiments. R1, R2 and R3 were sorted from triplicate experiments from pools of mice
Project description:Male and female collagen-enriched fraction of cartilage from bovine knees. Full thickness cartilage plugs without subchondral bone were digested with chondroitinase for 24 hours to remove proteoglycans. Cartilage was further decellularized with SDS.
LUM1_811145: channels 126, 127, 128 female, channels 129, 130, 131 male
LUM1_833734: channels 126, 127, 128 female, channels 129, 130, 131 male
Project description:Samples ECL1_1260429_F1-F8 are TMT6plex samples fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit. Samples are optic nerves protein lysates. Channels 126, 127, 128 are control samples (TFEBdPM-Halo) and channels 129, 130, 131 are mutant samples (Mog-Cre; TFEBdPM-Halo).
Samples 1251765_F1-F8 are TMT6plex samples fractionated into 8 fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit. Samples are day 14 in vitro differentiated oligodendrocytes protein lysates. Channels 126, 127, 128 are control samples (TFEBdPM-Halo) and channels 129, 130, 131 are mutant samples (Mog-Cre; TFEBdPM-Halo).
Project description:High toxin producing (HT) and a low toxin producing (LT) strains of Karenia brevis both derived from the Wilson strain.
LUM2_781143:
126: HT labeled before reduction with TCEP
127: LT labeled before reduction with TCEP
130: HT labeled after reduction with TCEP
131: LT labeled after reduction with TCEP
LUM2_781144:
128: HT labeled before reduction with TCEP
129: LT labeled before reduction with TCEP
130: HT labeled after reduction with TCEP
131: LT labeled after reduction with TCEP
LUM2_781145:
126: HT labeled before reduction with TCEP
127: LT labeled before reduction with TCEP
128: HT labeled after reduction with TCEP
129: LT labeled after reduction with TCEP
LUM2_800258:
126: HT labeled before reduction with TCEP
127: LT labeled before reduction with TCEP
129: LT labeled after reduction with TCEP
130: HT labeled after reduction with TCEP
Project description:The mouse liver mitochondrial proteome was analysed in four different mouse groups (allocation of the samples to Exp1 and Exp2 in brackets): AL (ad libitum fed) C57Bl6 (Exp1 129, Exp1 130, Exp2 130), DR (dietary restriction) C57Bl6 (Exp1 126, Exp1 127, Exp1 128), AL ICRFa (Exp2 128, Exp2 129) and old ICRFa (Exp2 126, Exp2 127). To achieve quantitative standardisation between Exp1 and Exp2, all 10 samples were pooled and one portion of the pool was anlaysed in each Exp (Exp1 131 and Exp2 131). Following tryptic digest and TMT 6-plex labelling, samples were separated by OffGel electrophoresis and all fractions were subsequently analysed by LCMSMS on an Orbitrap LTQ XL. One survey scan (res. 30,000) was followed by a high energy HCD scan (res. 6000) and a low energy CID scan in the LTQ. HCD data were used for the quantitation and CID data for the identification of peptides. Data were searched using Mascot (v. 2.2) through the Proteome Discoverer interface. Peptide raw quantitations were extracted as text files and further processed using dpeaqms (http://r-forge.r-project.org/projects/dpeaqms/) to obtain probability values for the differences in protein amounts for specific proteins between the different animal groups.
Project description:Arabidopsis thaliana mutant sr45-1 has an altered flower shape. sr45 is a splicing regulator. In this study, we examined the proteins from inflorescence of sr45-1 mutant plants and wild-type. Wild type TMT labels: 126, 128, 130. sr45-1 TMT labels: 127, 129, 131.
Project description:S-nitrosoproteome of leaves obtained from two rice cultivars Dongjin (relatively resistant) and Nipponbare (susceptible) to Magnaporthe oryzae strain PO6-6 was investigated using a iodo-TMT six plex kit. MS/MS data was acquired using two MS intruments: QExactive™ Orbitrap High-Resolution Mass Spectrometer (MS) (Thermo Fisher Scientific, MA, USA) or with Orbitrap Exploris™ 240 The labeling was performed in the following order: iodoTMT reagent 126: Dongjin mock iodoTMT reagent 127: Dongjin infected iodoTMT reagent 128: Dongjin mock+infected+GSNO iodoTMT reagent 129: Nipponbare mock iodoTMT reagent 130: Nipponbare infected iodoTMT reagent 131: Nipponbare mock+infected+GSNO