Proteomics

Dataset Information

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Halo-SPIN1_stable-cell-line_Halo-AP-MudPIT

ABSTRACT: Halo-SPIN1 stable cell line clone #9 & #11 in 293FRT cells Blank 293FRT control Halo purification Lys-C;Trypsin LTQ MudPIT

INSTRUMENT(S): LTQ

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Michael P. Washburn  

PROVIDER: MSV000084679 | MassIVE | Wed Dec 11 15:49:00 GMT 2019

REPOSITORIES: MassIVE

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Publications

Driving integrative structural modeling with serial capture affinity purification.

Liu Xingyu X   Zhang Ying Y   Wen Zhihui Z   Hao Yan Y   Banks Charles A S CAS   Lange Jeffrey J JJ   Slaughter Brian D BD   Unruh Jay R JR   Florens Laurence L   Abmayr Susan M SM   Workman Jerry L JL   Washburn Michael P MP  

Proceedings of the National Academy of Sciences of the United States of America 20201130 50

Streamlined characterization of protein complexes remains a challenge for the study of protein interaction networks. Here we describe serial capture affinity purification (SCAP), in which two separate proteins are tagged with either the HaloTag or the SNAP-tag, permitting a multistep affinity enrichment of specific protein complexes. The multifunctional capabilities of this protein-tagging system also permit in vivo validation of interactions using acceptor photobleaching Förster resonance energ  ...[more]

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