Mouse_Hippocampus_LCMSMS_IEF_Pooling

ABSTRACT: Protein extract from a single mouse hippocampus was enzymatically digested and fractionated by isoelectric focusing. Aliquots of fractions were pooled to make fewer, more complex samples. The unfractionated lysate, fractions, and pooled fractions were subjected to liquid chromatographyâ??mass spectrometry analysis. Samples consisting of many individual fractions had more protein identifications and quantified proteins with more spectral counts and greater precision than protein extract that was unfractionated or pooled into fewer LC-MS/MS samples.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Richard Nowakowski  

PROVIDER: MSV000084999 | MassIVE | Fri Feb 21 13:28:00 GMT 2020

SECONDARY ACCESSION(S): PXD002905

REPOSITORIES: MassIVE