Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

20-01068 Xiang et al cross-linking of antigen and nanobodies

ABSTRACT: Cross-linking studies of thirty six nanobodies with corresponding antigens. Either EDC or DSS was used in studies.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Recombinant Proteins

SUBMITTER: Yi Shi

PROVIDER: MSV000085117 | MassIVE | Wed Mar 18 11:24:00 GMT 2020

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

single step cross-linking ChIP-seq

Project description:single step cross-linking ChIP-seq

| PRJNA794167 | ENA

two steps cross-linking ChIP-seq

Project description:two steps cross-linking ChIP-seq

| PRJNA794157 | ENA

pH dependence of succinimide ester-based protein cross-linking for structural mass spectrometry applications

Project description:The pH dependency of the reaction of the cross-linking reagent, disuccinimidyl suberate (DSS), was studied on a set of eight model proteins.

2021-11-15 | PXD027891 | Pride

Comparison of OpenPepXL and xQuest on a Labeled Cross-Linking MS dataset

Project description:These data sets are part of a large study aimed at the comparison of experimental and computational workflows used in chemical cross-linking coupled to mass spectrometry. Bovine serum albumin (BSA) was used as a model protein. Two different cross-linking chemistries were used: disuccinimidyl suberate (DSS) and a combination of pimelic acid dihydrazide (PDH) and the coupling reagent, DMTMM. See related publication: Iacobucci et al., First Community-Wide, Comparative Cross-Linking Mass Spectrometry Study, Analytical Chemistry, 91 (2019) 6953-6961, DOI: 10.1021/acs.analchem.9b00658 This project is a reanalysis of these datasets with the two Labeled Cross-Linking MS identification tools OpenPepXL and xQuest.

2020-10-20 | PXD014523 | Pride

Two steps cross-linking LCL ChIP-seq

Project description:Two steps cross-linking LCL ChIP-seq

| PRJNA795780 | ENA

Single step cross-linking LCL ChIP-seq

Project description:Single step cross-linking LCL ChIP-seq

| PRJNA795781 | ENA

Cross-linking mass spectrometry analysis of human BCL7A and BAF47 in presence and absence of nucleosome

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the interaction between BCL7A and BAF47 and their respective interactions with nucleosomes. The two proteins were cross-linked with two different concentrations of disuccinimidyl suberate (DSS) in presence and absence of nucleosomes.

2025-04-14 | PXD054757 | Pride

Interactions of CDK19 with MED12 and cyclin-C assessed by chemical cross-linking mass spectrometry

Project description:Chemical cross-linking coupled to mass spectrometry was used to study binary and ternary complexes involving cyclin-dependent kinase 19 (CDK19), cyclin-C, and an N-terminal fragment of subunit 12 of the Mediator complex (MED12 1-100). Cross-linking was performed using disuccinimidyl suberate (DSS). These results were generated in the context of the study published as Klatt et al., A precisely positioned MED12 activation helix stimulates CDK8 kinase activity, Proc. Natl. Acad. Sci. USA 2020 (DOI: 10.1073/pnas.1917635117) with the associated data set PXD015394, but were not included in the article.

2020-05-18 | PXD019251 | Pride

Folding of beta-tubulin by the chaperonin TRiC/CCT studied by chemical cross-linking and mass spectrometry

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the folding of the client protein, beta-tubulin, by the chaperonin TRiC/CCT. Different complexes containing TRiC/CCT and/or the chaperone prefoldin were cross-linked in absence or presence of nucleotides with the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2022-12-08 | PXD030590 | Pride

Cross-linking mass spectrometry of a complex between the chaperonin TRiC/CCT and its cochaperones, prefoldin and phosducin-like protein 2A

Project description:Chemical cross-linking coupled to mass spectrometry was used to study the architecture of the co-complex between TRiC/CCT, PFD and PhLP2A. The complex was cross-linked with the homobifunctional, noncleavable reagent, disuccinimidyl suberate (DSS).

2023-12-11 | PXD040144 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data