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Top-down analysis of histone proteins from asynchronous chlamydomonas

ABSTRACT: These are salt-and-acid histone extracts from asynchronously grown Chlamydomonas measured by LC-MS/MS via top-down analysis. These 5 runs are biological repeats used in our JASMS 2021 paper (Rommelfanger et al.).

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Chlamydomonas Reinhardtii (ncbitaxon:3055)

SUBMITTER: Jim Pesavento

PROVIDER: MSV000088458 | MassIVE | Fri Nov 26 13:37:00 GMT 2021

REPOSITORIES: MassIVE

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An Improved Top-Down Mass Spectrometry Characterization of <i>Chlamydomonas reinhardtii</i> Histones and Their Post-translational Modifications.

Rommelfanger Sarah R SR Zhou Mowei M Shaghasi Henna H Tzeng Shin-Cheng SC Evans Bradley S BS Paša-Tolić Ljiljana L Umen James G JG Pesavento James J JJ

Journal of the American Society for Mass Spectrometry 20210624 7

We present an updated analysis of the linker and core histone proteins and their proteoforms in the green microalga <i>Chlamydomonas reinhardtii</i> by top-down mass spectrometry (TDMS). The combination of high-resolution liquid chromatographic separation, robust fragmentation, high mass spectral resolution, the application of a custom search algorithm, and extensive manual analysis enabled the characterization of 86 proteoforms across all four core histones H2A, H2B, H3, and H4 and the linker h ...[more]

PMID: 34165968

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