Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Untargeted_Sept_Nov_Carnitine_Study

ABSTRACT: 4-week-old male mice were infected with 50,000 CL+luc parasites or remained uninfected. Urine was collected pre-infection and 4 days after infection. 7 days post infection, mice were treated with 0.06% carnitine in drinking water ad libitum or with benznidazole 100 mg/kg ip for 10 days. 17 days post infection, urine was collected and euthanized. Metabolites were extracted from urine in methanol and untargeted LCMS analysis ran on a Q Exactive plus instrument using a Phenomenex Luna Omega Polar C18 column.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Laura Isobel McCall

PROVIDER: MSV000089114 | MassIVE | Wed Mar 23 16:07:00 GMT 2022

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Similar Datasets

Targeted_PRM_Urine_Carnitine_Efficacy_Study_Males_Females

Project description:5-week old male and female Swiss Webster mice were infected with 500,000 Trypanosoma cruzi strain Sylvio X/104 parasites. Urine was collected from uninfected mice pre-infection, from infected and uninfected mice every week for 5 weeks post infection, from infected and uninfected mice 67 (females)/68 (males) days post infection, and from infected and uninfected mice 117 (females)/124 (males) days post infection for acute, mid-chronic, and chronic time-points. Metabolites were extracted with methanol and a targeted PRM LCMS analysis was ran on a Thermo Q Exactive Plus instrument with a Phenomenex Luna Omega Polar C18 column.

2022-03-23 | MSV000089113 | MassIVE

Untargeted_Urine_Carnitine_Efficacy_Study_Males_Females

Project description:5-week old male and female Swiss Webster mice were infected with 500,000 Trypanosoma cruzi strain Sylvio X10/4 parasites. Urine was collected from uninfected mice pre-infection, from infected and uninfected mice every week for 5 weeks post infection, from infected and uninfected mice 67 (females)/68 (males) days post infection, and from infected and uninfected mice 117 (females)/124 (males) days post infection for acute, mid-chronic, and chronic time-points. Metabolites were extracted with methanol and an untargeted LCMS analysis was ran on a Thermo Q Exactive Plus instrument with a Phenomenex Luna Omega Polar C18 column.

2022-03-23 | MSV000089112 | MassIVE

GNPS-carnitine treatment, acute stage Chagas disease, liver and oesophagus, positive mode

Project description:Male C3H/HeJ mice were infected with 50,000 luciferase-expressing strain CL Brener trypanosoma cruzi parasites. Beginning 7 days post-infection, mice were treated with carnitine in drinking water (100 mg/kg/day dosing equivalent), benznidazole (100 mg/kg/day by intraperitoneal injection), or left untreated (vehicle group). One additional control group was uninfected. Mice were euthanized and liver and oesophagus collected at day 17 post-infection. Tissue was extracted with 50% methanol followed by 3:1 dichloromethane:methanol, and analyzed by C8 chromatography, with detection by MS in positive mode.

| MSV000084325 | GNPS

An evaluation of the Clear Fluids Diet: A comparison between patients commencing on Clear Fluids or Free Fluids and the effect this has on post-operative outcomes after elective large bowel surgery.

Project description:Interventions: Patients commence on sips of water ad libitum day 1 post-operatively. Once the treating surgeon deems the patient appropriate to commence fluids, they will start on Free Fluids ad libitum, (usually for 1-4 days), then Light Diet ad libitum (1-2 days) and then to Full Diet ad libitum (until discharged) Free Fluids are defined as low fibre foods which are fluid like in nature at room temperature including, all Clear Fluids, milk and soy based drinks and nutrition supplements, strained porridge, pureed soup, ice-cream, plain yoghurt and custard Primary outcome(s): Number of days of hospital admission post surgery collected from medical records.[At discharge from Frankston Hospital];Post-operative complications including ileus, anastomotic breakdown and wound breakdown as documented in the medical history by the medical team. Pneumonia (from chest xray showing positive infiltrates and positive sputum culture), urinary tract infection (positive urine cultures), sepsis (temperature greater than 38 degrees, heart rate above 90 bpm, respiratory rate greater that 20 rpm and white cell count above 12 x10^9/L or less than 4 x10^9/L or more than 10 % band forms in the absence of chemotherapy induced neutropenia or leukopenia) and mortality from medical history.[Incidence of complications will be collected during hospital admission];Proportion of nutritional requirements met using food and fluid chart analysis. Nutritional requirements will be calculated using the Schofield Equation.[Until patient is tolerating Light Diet for 24 hours] Study Design: Purpose: Treatment; Allocation: Randomised controlled trial

| 2455275 | ecrin-mdr-crc

Small RNA Sequencing facilitates quantitative analysis of miRNA expression in feces from mice during DSS-induced colitis and the recovery phase

Project description:Mice were exposed to 2.5% DSS (MW 36,000–50,000, MP Biomedicals) prepared in autoclaved drinking water for five days, after which they were provided DSS-free drinking water for 13 days. On day 12, 16.7% of mice (20 of 120) whose body weight returned to the same level as on day 1 (the change of body weight was less than 0.5 g) and exhibited an DAI of 0 or 1.00, were used as “well-recovered” mice. To identify which fecal miRNAs were affected during DSS-induced colitis and the recovery phase, we performed small RNA sequencing in feces obtained from well-recovered mice on days 0, 5, and 12 of the treatment regimen.

2021-01-28 | GSE165648 | GEO

Impact of Nosema apis infection on worker honey bee midgut gene expression

Project description:Newly emerged adult workers (24 hours old) were infected with 50,000 Nosema apis spores in sucrose solution. Controls were fed sucrose. Workers were maintained in cages in an incubator and collected at 1 and 2 days post-infection. Midguts were dissected and whole genome expression in this tissue was compared across treatments and collection time points using microarrays.

2014-05-03 | E-MEXP-3891 | biostudies-arrayexpress

Impact of Nosema apis infection on worker honey bee fat body gene expression

Project description:Newly emerged adult workers (24 hours old) were infected with 50,000 Nosema apis spores in sucrose solution. Controls were fed sucrose. Workers were maintained in cages in an incubator and collected at 2 and 7 days post-infection. Fat body tissue was dissected (eviscerated abdomen) and whole genome expression in this tissue was compared across treatments and collection time points using microarrays.

2014-05-03 | E-MEXP-3892 | biostudies-arrayexpress

GNPS-acute stage Chagas disease carnitine treatment, large intestine (negative mode)

Project description:Large intestine samples from mice infected with 50,000 Trypanosoma cruzi parasites or left uninfected. One week post-infection, mice were treated with carnitine, benznidazole or vehicle. Animals were euthanized after 10 days of treatment and organs collected. Metabolites were extracted with 50% methanol followed by 3:1 dichloromethane-methanol and analyzed by C8 chromatography, with negative mode ddMS2 data collection (data-dependent).

2020-03-24 | MSV000085143 | GNPS

GNPS-acute stage Chagas disease carnitine treatment, large intestine (positive mode)

Project description:Large intestine samples from mice infected with 50,000 Trypanosoma cruzi parasites or left uninfected. One week post-infection, mice were treated with carnitine, benznidazole or vehicle. Animals were euthanized after 10 days of treatment and organs collected. Metabolites were extracted with 50% methanol followed by 3:1 dichloromethane-methanol and analyzed by C8 chromatography, with positive mode ddMS2 data collection (data-dependent).

2020-03-24 | MSV000085142 | GNPS

Analysis of 20-HETE supplementation in calorically restricted C57Bl6 mice

Project description:Proteomic dataset of 15 murine kidneys of 14-week-old male C57Bl6 mice. Mice were divided into 3 treatment groups four weeks prior to nephrectomy and 5 mice had ad libitum access to food and water, while another 10 mice received 30% food restriction. Calorically restricted mice either received intraperitoneal supplementation of 20-HETE or vehicle (EtOH in sodiumchloride 0.9%) for 8 days on a daily basis prior to nephrectomy. Ad libitum fed mice received vehicle injections for 8 days on a daily basis prior to nephrectomy.

2022-06-17 | PXD031604 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data