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Amyloid fibril polymorphism in the heart of an ATTR amyloidosis patient with polyneuropathy attributed to the V122delta variant

ABSTRACT: We have aggregated protein (fibrils, heart) at a total amount of 1ug. Proteins were run on SDS page gel for 10 mins, then the gel was stained with using Coomassie Blue for 1h. Protein smear is cut and in-gel digestion performed with trypsin. Data analysis include search for both tryptic and semi tryptic fragments, Transthyretin has a heterozygous mutation of V122delta, so we expect to see some fragments with and without V122.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Lorena Saelices Gomez

PROVIDER: MSV000094731 | MassIVE | Thu May 09 14:07:00 BST 2024

REPOSITORIES: MassIVE

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