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Phenotypic screening against P. falciparum under physiologically relevant nutrients levels reveals novel chemical diversity and highlights the importance of the PSAC channel for parasite survival.

ABSTRACT: Microenvironment mapping was used to identify the binding targets of two small molecules in Plasmodium Falciparum cells. In the method, small molecule - photocatalyst conjugates sensitize biotinylation of proteins binding the small molecules, which were reduced, alkylated with iodoacetamide, and immunoprecipitated using streptavidin beads. Labeling was subject to offcompete using a large excess of the free small molecule. On-bead tryptic digestion yielded peptides, which were subjected to DDA-PASEF MS measurement using a timsTOF Pro 2. CLAG3 was significantly enriched by labeling by both molecules.

INSTRUMENT(S): timsTOF Pro 2

ORGANISM(S): Plasmodium Falciparum (ncbitaxon:5833)

SUBMITTER: Jacob Geri

PROVIDER: MSV000095249 | MassIVE | Thu Jul 04 09:34:00 BST 2024

SECONDARY ACCESSION(S): PXD053644

REPOSITORIES: MassIVE

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