Proteomics

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QCeltis Case Study 3: Dried Blood Spots QC Dataset

ABSTRACT: A dataset containing dried blood spots from human blood used as quality control samples to monitor variation, used as technical replicates (TR), digestion replicates (DR) and HeLa samples. Dried blood spots are blood from a finger prick spotted onto Whatman cards and dried. An aliquot of pooled, mixed-gender blood sample (Golden West Biosolutions, LLC., Human Whole Blood Lot# PS1000.104) were spotted on the same Whatman cards and used as DRs. 6 DRs were added to each digestion plate to be digested with the cohort data. During LC-MS acquisition, the 6 DRs are run at the beginning of each batch. In addition, the pooled, mixed-gender blood samples were bulk digested and included as TRs. 2 TRs were run for every acquisition batch, with the goal of assessing LC-MS performance. To further this goal, 2 HeLa samples were also run between every 2 MS batches.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Jennifer Van Eyk  

PROVIDER: MSV000095745 | MassIVE |

SECONDARY ACCESSION(S): PXD055484

REPOSITORIES: MassIVE

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Publications

<u>Enhancing Proteomics Quality Control: Insights from the Visualization Tool QCeltis</u>.

Vegesna Manasa M   Sundararaman Niveda N   Bharadwaj Ajay A   Washington Kirstin K   Pandey Rakhi R   Haghani Ali A   Chazarin Blandine B   Binek Aleksandra A   Fu Qin Q   Cheng Susan S   Herrington David D   Van Eyk Jennifer E JE  

Journal of proteome research 20250224 3

Large-scale mass-spectrometry-based proteomics experiments are complex and prone to analytical variability, requiring rigorous quality checks across each step in the workflow: sample preparation, chromatography, mass spectrometry, and the bioinformatics stages. This includes quality control (QC) measures that address biological and technical variation. Most QC approaches involve detecting sample outliers and monitoring parameters related to sample preparation and mass spectrometer performance. E  ...[more]

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