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ABSTRACT: Target capture sequencing using dried blood spots (DBS) samples for viral screening from wildlife animals

PROVIDER: PRJNA1024660 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR26299345_1.fastq.gz	Fastqsanger.gz
	SRR26299345_2.fastq.gz	Fastqsanger.gz
	SRR26299346_1.fastq.gz	Fastqsanger.gz
	SRR26299346_2.fastq.gz	Fastqsanger.gz
	SRR26299347_1.fastq.gz	Fastqsanger.gz

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High Field Asymmetric Waveform Ion Mobility Spectrometry in Non-targeted Bottom-up Proteomics of Dried Blood Spot

Project description:Dried Blood Spots samples are a rich source of proteins and have therefore great potential for proteomics biomarker discovery studies. Only a few articles have used DBS samples for non-targeted bottom-up protein analysis, probably due to the complexity of the DBS samples. Gas-phase separation by ion mobility spectrometry such as Field Asymmetric Waveform Ion Mobility (FAIMS) could be useful in analysis of DBS samples as FAIMS has previously shown to be advantageous for bottom-up protein analysis of complex samples. The aim of this project was therefore to evaluate FAIMS in non-targeted analysis of Dried Blood Spots.

2018-05-03 | PXD007926 | Pride

Visual automated fluorescence electrophoresis provides simultaneous quality, quantity, and molecular weight spectra for genomic DNA from archived neonatal blood spots

Project description:The Guthrie 903 card archived dried blood spots (DBS) are a unique but terminal resource amenable for individual and population wide genomic profiling. The limited amounts of DBS-derived genomic DNA (gDNA) can be whole-genome amplified (WGA) producing sufficient gDNA for genomic applications, albeit with variable success, and optimizing the isolation of high-quality DNA from these finite, low-yield specimens is essential. Visual automated fluorescence electrophoresis (VAFE) is a novel QC technology affording precise quality, quantity and molecular weight of double-stranded DNA from a single microliter of sample. The VAFE QC data were correlated with subsequent sample performance in PCR, sequencing, and high-density comparative genome hybridization array. The Swedish Repository of DBS samples collected on Guthrie 903 cards for neonatal screening of inborn diseases provided deidentified 3mm blood spot punches. There were total of eight (8) samples representing two (2) decades; 1970s: 1975, 1976, 1977, 1978; 1980s: 1980, 1982, 1984, 1986. gDNA was extracted and whole genome amplified prior to aCGH experiments using control female reference genomic DNA. The objective was to show that large rearrangements (e.g. loss of chrX in male samples) can be detected in WGA gDNA from blood spots >30 years old.

2013-07-01 | E-GEOD-44249 | biostudies-arrayexpress

Visual automated fluorescence electrophoresis provides simultaneous quality, quantity, and molecular weight spectra for genomic DNA from archived neonatal blood spots

2013-07-01 | GSE44249 | GEO

Multigenerational epigenetic inheritance in humans: DNA methylation changes associated with maternal exposure to lead can be transmitted to the grandchildren

Project description:We report that the DNA methylation profile of a child’s neonatal whole blood can be significantly influenced by his or her mother’s neonatal blood lead levels (BLL). We recruited 35 mother-infant pairs in Detroit and measured the whole blood lead (Pb) levels and DNA methylation levels at over 450,000 loci from current blood and neonatal blood from both the mother and the child. We found that mothers with high neonatal BLL correlate with altered DNA methylation at 564 loci in their children’s neonatal blood. Our results suggest that Pb exposure during pregnancy affects the DNA methylation status of the fetal germ cells, which leads to altered DNA methylation in grandchildren’s neonatal dried blood spots. This is the first demonstration that an environmental exposure in pregnant mothers can have an epigenetic effect on the DNA methylation pattern in the grandchildren. For the study, we selected 35 dried blood spots (DBS) collected from mother-infant pairs from Health Fairs ran in three Detroit communities, because they have a high prevalence (8-11%) of high BLL in children. The sample set consisted of 25 male children and 18 female children. We also collected the neonatal DBS and mother neonatal DBS for these mother-infant pairs from the Michigan Neonatal Biobank. Then we measured a blood lead levels in dried blood spots using using atomic absorbtion spectrophotometry. Finally we measured the DNA methylation levels using human methylation 450K array from Illumina. Then we normalized the data for technical biases and tried to infer the the locus specific DNA methylation changes due to Pb exposure which was carried over from the grandmothers to the grandchildren by the Pb –exposed fetal germ cells of the mother using statistical model proposed by Sofer et al, 2012.

2015-04-01 | E-GEOD-67444 | biostudies-arrayexpress

Development of non-targeted proteome analysis of dried blood spots using an automated protein extraction system (

Project description:In this study, we developed a simple protein extraction method of dried blood spots (DBS) that potentially meets the throughput required for newborn screening (NBS) and optimized non-targeted proteomic analysis using this method in combination with liquid chromatography coupled mass spectrometry in data-independent-acquisition mode (DIA-LC-MS/MS).

2025-08-14 | PXD064701 | JPOST Repository

Evaluation of suitability of dried saliva spots for in-depth proteome analyses of clinical applications - Additional file

Project description:We performed a single-shot DIA-MS proteome analysis of dried saliva spots (DSS) to investigate whether it provides a platform to complement the dried blood spot (DBS) data.

2022-04-26 | PXD032116 | JPOST Repository

Evaluation of suitability of dried saliva spots for in-depth proteome analyses of clinical applications

Project description:We performed a single-shot DIA-MS proteome analysis of dried saliva spots (DSS) to investigate whether it provides a platform to complement the dried blood spot (DBS) data.

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Victorian Infant Collaborative Study

Project description:DNA samples were derived from dried blood spots taken for newborn screening when infants were several days of age, after obtaining permission from the participants when they were aged 18 years, or from their parents if they were younger than 18 years.

2016-04-18 | GSE80283 | GEO

Supporting data for manuscript: A modified fully automated dried blood spot sample preparation system and its application in steroid esters detection

Project description:This project focuses on developing an efficient and sensitive method for detecting steroid esters in dried blood spots (DBS) to support anti-doping efforts. By optimizing a fully automated DBS processing system with an independent desorption pump, we established a high-throughput screening method for 30 steroid esters and a confirmatory method for 26 targets using HPLC-HRMS. The method demonstrates low detection limits (as low as 0.05 ng/mL) and good reproducibility (RSD < 15%), and its performance was validated through blind samples, administration studies, and comparison with traditional IRMS. This approach provides a practical solution for rapid and accurate screening and confirmation of steroid esters, enhancing anti-doping capabilities.

2025-08-20 | PXD067516 |

Epigenome wide DNA methylation profile of neonatal blood spots from the Archives for Research on Child Health (ARCH) cohort

Project description:Genome wide DNA methylation profiling of human samples from dried neonatal blood spots taken at birth from a cohort of pregnant people and their infants in Michigan. The Illumina MethylationEPIC Beadchip array was used to obtain DNA methylation profiles across over 850,000 CpGs in dried neonatal blood spot samples. Samples were collected from 166 infants at birth.

2022-01-31 | GSE195595 | GEO

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