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Human Platelet Proteome and Site-Specific Glycoproteome analysis

ABSTRACT: The understanding of peripheral blood platelets as indicators of cancer progression and the significance of abnormal glycosylation in platelet function and related disorders are increasingly recognized. In this study, our analysis of a large cohort of liver cancer patients revealed decreased PLC and elevated PT as poor prognostic factors, emphasizing the association between platelets and liver cancer progression. Herein, we conducted platelet proteome and site-specific glycoproteome analysis, generating a dataset comprising 3,466 proteins with qualitative information and 3,199 proteins with quantitative information, and 3,419 site-specific glycans with qualitative information and 3,377 site-specific glycans with quantitative information from peripheral blood platelets of 30 HCC patients, 30 metastatic liver cancer patients, and 16 healthy controls. Integrated analysis revealed significant changes in platelet protein N-glycosylation in liver cancer patients. Further systems biology analysis and lectin pull-down-coupled ELISA assays in independent clinical samples confirmed two N-glycoproteins with specific glycan types, CO3 with high-mannose modification and ITB3 with sialylation, as potential biomarkers distinguishing normal individuals from liver cancer patients, highlighting the potential of platelet glycosylated proteins as biomarkers.

INSTRUMENT(S): Orbitrap Eclipse, timsTOF Pro

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Weiqian Cao

PROVIDER: MSV000096240 | MassIVE | Tue Oct 29 04:31:00 GMT 2024

REPOSITORIES: MassIVE

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