Dataset Information

Hepatic p62, a major autophagic receptor in CYP4A ALD

ABSTRACT: In cell DSS crosslinking of HEK293 cells expressing His-tagged CYP4A11 and HA-tagged p62. Cells were treated with DSS, lysed, and enriched by Ni-NTA affinity chromatography. The eluate was separated by SDS-PAGE and the CYP-p62 crosslinked band identified by multi-channel western blot visualization. The crosslinked band was excised and in gel digested. Crosslinked peptides were analyzed on an Orbtrap Exploris 480 with a FAIMS source and running a 0.75 um x 50 cm c18 column with a 4-hour separation. The sample was injected twice using two different sets of compensation voltages: S20240820-04.raw: -45, -60, -70 V. S20240820-05: -40, -50, -65C.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Maria Almira Correia