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Establishment of a murine model of beta-parvalbumin-induced fish allergy and proteomic profiling of immune cell activation using DIA-based ZenoSWATH-MS

ABSTRACT: Food allergy is an immune-mediated condition caused by hypersensitivity to food allergens, with fish allergens, particularly beta-parvalbumins (beta-PRVBs), being the most significant. While the only effective treatment is avoidance of the food allergen, the intracellular mechanisms of immune activation during allergen exposure are not well understood. This study aimed to investigate critical insights into immune cell activation in a newly proposed beta-PRVB-induced fish allergy model, with a particular focus on to explore the proteomic phenotype of splenocytes and T cell activation in response to the allergen. To establish a robust allergic response, mice were sensitized to beta-PRVB using aluminium hydroxide, a Th2-skewing adjuvant, (intraperitoneal injection) and subsequently challenged orally with beta-PRVB . This carefully designed protocol was designed to induce a Th2-type immune response, which was confirmed by detecting allergen-specific antibodies and cytokines. For proteomic analysis of the immune response, high-resolution quantitative proteomics data were generated using an advanced DIA-based ZenoSWATH-MS technology.

INSTRUMENT(S): ZenoTOF 7600

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Monica Carrera

PROVIDER: MSV000097859 | MassIVE | Tue May 13 13:28:00 BST 2025

REPOSITORIES: MassIVE

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