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Mitochondrial Complex I in the diatom Phaeodactylum tricornutum

ABSTRACT: Diatoms are among the most ecologically successful microalgae, contributing significantly to marine primary production and global carbon cycling. Despite their ecological importance, detailed proteomic characterization on diatoms has remained limited. Here, we report a method for the isolation of mitochondrial complex I from P. tricornutum and present a comprehensive proteomic analysis. We create two GelMaps allowing identification of protein complex subunits from a organelle fraction as well as from a complex I enriched fraction. Our results confirm the presence of a carbonic anhydrase (CA) module, previously proposed as an ancestral feature of complex I, and identify one novel, clade-specific subunit that may contribute additional functionality.

INSTRUMENT(S): timsTOF Pro, Q Exactive

ORGANISM(S): Phaeodactylum Tricornutum (ncbitaxon:2850)

SUBMITTER: Hans-Peter Braun

PROVIDER: MSV000098949 | MassIVE | Wed Aug 27 06:08:00 BST 2025

SECONDARY ACCESSION(S): PXD067795

REPOSITORIES: MassIVE

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