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The Organ_resolved Metabolome Atlas of the Aging Mice_HILIC_POS and NEG

ABSTRACT: We profiled polar metabolites across multiple organs and fluids from C57BL/6NCrl (B6NCrl-MVP) wild-type mice at two ages (16 and 92 weeks), including both sexes. Samples spanned the nervous (hippocampus, cerebrospinal fluid), connective (brown, subcutaneous, and visceral/abdominal fat), digestive (stomach, duodenum, jejunum, ileum, cecum, colon, liver, gallbladder), immune (spleen, thymus), reproductive (testes, uterus), excretory (kidney, bladder), endocrine (pancreas), cardiovascular (heart), muscular (quadriceps), and respiratory (lung) systems, plus biofluids (plasma, urine) and digestive products (feces). Polar metabolomics was performed by LC-MS/MS on a Thermo Scientific Q Exactive HF-X Quadrupole-Orbitrap coupled to a Vanquish UHPLC in positive mode. Metabolites were separated on a Waters ACQUITY Premier BEH Amide column (50 x 2.1 mm, 1.7 um) prior to high-resolution MS acquisition.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Oliver Fiehn

PROVIDER: MSV000099387 | MassIVE | Sun Oct 05 11:51:00 BST 2025

REPOSITORIES: MassIVE

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