Anti-C-RAF IP-MS in MG-treated NCI-H226 cells
Ontology highlight
ABSTRACT: NCI-H226 cells in 100 mm dishes were treated with 10 uM of compounds 22 or 23 or DMSO at equal volume (final [DMSO] = 0.02%) for 24 h in triplicates for each condition (n = 3). Soluble lysates proceeded to anti-endogenous CRAF IP-MS with anti-CRAF antibody (BD Biosciences 610152; incubated at 4C overnight) and protein A/G beads (Pierce 88802; incubated at RT for 1 h), and on-bead digestion with PreOmics sample cleanup kit. 200 ng of peptides were quantified and injected onto Orbitrap Eclipse with 60 min gradient using DIA.
INSTRUMENT(S): Orbitrap Eclipse
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER:
Michelle Arkin
PROVIDER: MSV000099423 | MassIVE | Wed Oct 08 12:15:00 BST 2025
SECONDARY ACCESSION(S): PXD069213
REPOSITORIES: MassIVE
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